Expression and Functional Analysis of the BCL2-Associated Agonist of Cell Death (BAD) Gene in the Sheep Ovary During the Reproductive Cycle.
Expression and Functional Analysis of the BCL2-Associated Agonist of Cell Death (BAD) Gene in the Sheep Ovary During the Reproductive Cycle.
复制标题
绵羊卵巢中 BCL2 相关细胞死亡激动剂 (BAD) 基因的表达及功能分析
DOI：
10.3389/fendo.2018.00512
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发表时间：
2018
影响因子：
5.2
通讯作者：
Chu M
中科院分区：
文献类型：
--
作者：
Cao X;Wang X;Lu L;Li X;Di R;He X;Hu W;Zeng X;Liu Q;Chu M
中国的大多数母羊是季节性多排卵的，排卵周期正常，受光周期（日光照期的长度）控制。这些母羊在短日照季节发情，在长日照季节发情，在发情期间不能交配。因此，季节性繁殖限制了生产的多样化和集约化。如果羊能全年发情，每年可配种两次，可大大提高经济效益。了解绵羊季节性繁殖性状的分子机制，对于在遗传水平上改变绵羊季节性发情，培育绵羊新品种具有重要意义。BCL 2相关的细胞死亡激动剂（BAD）基因是细胞凋亡的调节剂，其通过我们先前的RNA-Seq鉴定，其与哺乳动物卵巢中的卵泡发育相关。然而，BAD如何调节绵羊发情的机制还知之甚少。本研究对绵羊BAD基因进行了研究，包括全长mRNA的克隆和蛋白质序列预测，以及BAD基因在小尾寒羊中的表达谱。BAD在绵羊下丘脑、肺和垂体中的表达水平最高，而在肝脏中的表达水平最低。在绵羊的初级颗粒细胞中进行了BAD的功能分析。在体外实验中，BAD基因的RNAi干扰后，P4的表达水平显著升高，而BAD基因的过表达则相反。研究发现，BAD可通过促进卵巢GC凋亡降低孕酮水平，可能参与绵羊发情周期的调控。
Most ewes in China are seasonally polyestrous with normal ovulatory cycles, which is controlled by photoperiod (length of the daily light phase). These ewes are estrous in the short-day season and anestrus in the long-day season and cannot mate during anestrus. Thus seasonal breeding limits both diversification and intensification of production. If sheep can estrus all round year, it can be mated twice per year, which can greatly improve the economic benefits. To change seasonal estrus at the genetic level and cultivating new sheep breeds, it is important to understand the molecular mechanisms of seasonal breeding trait in sheep. The BCL2-associated agonist of cell death (BAD) gene being a regulator of cellular apoptosis was identified by our previous RNA-Seq, which is associated with follicular development in mammalian ovaries. However, the mechanism how BAD can regulate estrus in sheep was poorly understood. In this study, we characterized ovine BAD, including full-length mRNA cloning and protein sequence prediction, as well as BAD expression profile in Small-tailed Han (STH) sheep. The highest expression levels of BAD were observed in sheep hypothalamus, lung, and pituitary, while the lowest expression was in liver. Functional analysis of BAD was performed in primary granulosa cells of sheep. The concentration of P4 was significantly increased after RNAi interference of BAD, while P4 level was shown to be opposite after BAD overexpression in vitro. It has been found that BAD can reduce progesterone levels by promoting ovarian GC apoptosis, which might be involved in regulating the estrus cycle in sheep.