Marker enrichment and construction of haplotype-specific BAC contigs for the polyembryony genomic region in Citrus
Marker enrichment and construction of haplotype-specific BAC contigs for the polyembryony genomic region in Citrus
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DOI：
10.1270/jsbbs.58.375
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发表时间：
2008-12-01
期刊：
影响因子：
2.4
通讯作者：
Omura, Mitsuo
中科院分区：
文献类型：
--
作者：
Nakano, Michiharu;Shimizu, Tokurou;Omura, Mitsuo
多胚性，即合子胚胎和无融合生殖胚胎在同一种子中同时发育，在柑橘中广泛存在。为了获得更多关于多胚性基因座先前特征图谱位置的基因组信息，利用多胚性基因座两侧的共同标记确定了来自3个不同杂交群体的连锁图谱之间的关系。利用裂解扩增多态序列(CAPS)标记在F180בHarumi’群体中定位多胚性基因座，在与标记Mf0086共分离的高密度标记区域检测到多胚性基因座。利用分子标记Mf0086筛选细菌人工染色体(BAC)文库，构建了反映多胚胎和单胚胎单倍型的重叠群。检测到的单核苷酸多态(SNP)标记将这些重叠群定位在‘宫川大米’和‘春美’连锁群I中距离多胚点最近的位置。这些不同亲本的多胚性品种多胚性基因侧翼区域的高度保守的标记谱系表明，多胚性基因的基因组区域在许多柑橘品种中可能具有共同的结构。
Polyembryony, in which zygotic and apomictic embryos develop concurrently in a seed, is widespread in Citrus. To obtain further genomic information about the previously characterized map position of the polyembryony locus, the relationship among linkage maps from 3 different hybrid populations was determined using common markers flanking the polyembryony locus. By mapping the polyembryony locus With cleaved amplified polymorphic sequence (CAPS) markers in a F180 x 'Harumi' population, the polyembryony locus was detected in a high-density marker region co-segregated to marker Mf0086. By screening a bacterial artificial chromosome (BAC) library with marker Mf0086, two contigs reflecting the polyembryony and monoembryony haplotype were constructed. The single nucleotide polymorphism (SNP) markers detected oil these contigs were mapped oil the nearest position to the polyembryony locus in linkage group I of 'Miyagawa wase' and 'Harumi'. The highly conserved marker lineage in the flanking regions of the polyembryony locus in these polyembryonic cultivars of different parentage suggested that the genomic region of the polyembryony locus might have a common structure in a wide range of Citrus cultivars.