Functional analysis of histone deacetylase and its role in stress response, drug resistance and solid-state cultivation in Aspergillus oryzae
Functional analysis of histone deacetylase and its role in stress response, drug resistance and solid-state cultivation in Aspergillus oryzae
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DOI：
10.1016/j.jbiosc.2014.02.004
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发表时间：
2014-08-01
影响因子：
2.8
通讯作者：
Iwashita, Kazuhiro
中科院分区：
文献类型：
--
作者：
Kawauchi, Moriyuki;Iwashita, Kazuhiro
在真核细胞中，组蛋白去乙酰化酶（HDAC）在发育调控、应激反应、次生代谢和基因组完整性等基本细胞过程中起着重要作用。在这里，我们提供了一个全面的表型分析，使用HDAC破坏曲霉。结果表明，hdaA/Aohda 1、hdaB/Aorpd 3、hdaD/Aohos 2和hst 4/AohstD四种HDAC参与了拟南芥的胁迫反应、细胞壁合成和染色质完整性。米。HDAC干扰物的渗透胁迫敏感性在平板培养和液体培养之间存在差异，表明HDAC适应不同的环境条件。用一个普通的A.除了发酵培养基，米曲，我们还表征了与生长和酶产生相关的HDAC，以研究HDAC对环境条件和胁迫抗性的适应性。由于HDAC广泛保守，我们的研究具有广泛的应用，并可能为丝状真菌和其他真核生物的工作提供信息。(C)2014年，生物技术学会，日本。All rights reserved.
In the eukaryotic cell, histone deacetylases (HDACs) play key roles in the regulation of fundamental cellular process such as development regulation, stress response, secondary metabolism and genome integrity. Here, we provide a comprehensive phenotypic analysis using HDAC disruptants in Aspergillus oryzae. Our study revealed that four HDACs, hdaA/Aohda1, hdaB/Aorpd3, hdaD/Aohos2 and hst4/AohstD were involved in stress response, cell wall synthesis and chromatin integrity in A. oryzae. Osmotic stress sensitivity of HDAC disruptants differed between plate cultures and liquid cultures, suggesting that HDACs adapt to the difference environmental conditions. Using a common A. oryzae fermentation medium, rice-koji, we also characterized HDACs related to growth and enzyme production to investigate which HDACs will be required for adaptation to environmental conditions and stress resistances. Because HDACs are widely conserved, our study has broad applications and may inform work with filamentous fungi and other eukaryote. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.