Isolation and identification of novel microRNAs from Marsupenaeus japonicus
Isolation and identification of novel microRNAs from Marsupenaeus japonicus
复制标题
日本袋对虾新型 microRNA 的分离与鉴定
DOI：
10.1016/j.fsi.2011.05.023
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发表时间：
2011-08-01
影响因子：
4.7
通讯作者：
Yan, Xiumin
中科院分区：
文献类型：
--
作者：
Ruan, Lingwei;Bian, Xiaofang;Yan, Xiumin
MicroRNAs （miRNAs）是一类小的非编码rna，其功能是调节基因表达。它们在各种生物过程中发挥重要作用，如发育、分化和免疫反应。在本研究中，我们从日本有袋鳅中鉴定出35个mirna。其中15个mirna与其他节肢动物的已知mirna具有高度同源性，其余可能是新的mirna。我们进一步发现了WSSV感染与22种mirna表达水平的相关性。这是首次从虾中鉴定出mirna。我们的研究结果扩展了对甲壳类动物基因调控的认识，为今后对虾病毒感染免疫的研究提供了线索。(C) 2011 Elsevier Ltd.版权所有。
MicroRNAs (miRNAs) are a class of small noncoding RNAs that function as regulators of gene expression. They play essential roles in various biological processes, such as development, differentiation and immune response. In this study, we identified 35 miRNAs from Marsupenaeus japonicus. Among them, fifteen miRNAs exhibited high homology to the known miRNAs from other arthropods, while the rest might represent novel miRNAs. We further showed a correlation of WSSV infection and the expression levels of 22 miRNAs. This is the first report to identify miRNAs from the shrimp. Our results extend the knowledge of the gene regulation of crustacean, providing clues for future researches of shrimp immunity against virus infection. (C) 2011 Elsevier Ltd. All rights reserved.