Transcriptome analysis of medaka (Oryzias latipes) exposed to tributyltin
Transcriptome analysis of medaka (Oryzias latipes) exposed to tributyltin
复制标题
暴露于三丁基锡的青鳉 (Oryzias latipes) 的转录组分析
DOI：
10.11403/jset.23.10
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Y. Oshima
中科院分区：
文献类型：
--
作者：
Yuki Takai;T. Takamura;Shintaro Enoki;Moeko Sato;Yoko Kato;Xuchun Qiu;Y. Shimasaki;Y. Oshima
三丁基锡（TBT）是一种有机锡化合物，可扰乱水生生物的内分泌系统，其对各种物种的致肥毒性众所周知。然而，三丁基锡化合物干扰内分泌系统的机制尚未澄清。因此，为了研究TBT对鱼类的影响，我们将青鳉（Oryzias latipes）幼鱼暴露于TBT，并使用mRNA-Seq分析基因表达的变化。分析结果表明，毒性相关基因，如与激素代谢相关的细胞色素P450超家族基因和与肥胖相关的过氧化物酶增殖物激活受体信号通路基因，明显受到三丁基锡化合物的影响。因此，我们的mRNA-Seq结果确定了参与TBT暴露青鳉中TBT毒性机制的候选基因。mRNA-Seq可能是研究和进一步了解污染物引起的毒性效应的有力工具。
Tributyltin (TBT) is an organotin compound that disrupts the endocrine system of aquatic organisms, and its obesogenic toxicity to various species is well known. However, the mechanism by which TBT disrupts the endocrine system has not been clarified. Therefore, to investigate the eects of TBT in fish, we exposed juvenile medaka (Oryzias latipes) to TBT and analyzed the gene expression changes using mRNA-Seq. As a result of this analysis, it was clear that toxicity-related genes, such as cytochrome P450 superfamily genes connected to hormonal metabolism, and peroxisomeproliferator-activated receptor signaling pathway genes related to obesity, were significantly aected by TBT. Thus, our mRNA-Seq results identified candidate genes for involvement in the mechanisms of TBT toxicity in TBT-exposed medaka. mRNA-Seq could be a strong tool to investigate and further understand the toxic effects caused by pollutants.
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影响因子：
64.8
作者：
Kasahara, Masahiro;Naruse, Kiyoshi;Kohara, Yuji
通讯作者：
Kohara, Yuji
影响因子：
14.9
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
14.9
作者：
Ogata, H;Goto, S;Kanehisa, M
通讯作者：
Kanehisa, M
影响因子：
5.8
作者：
Murai, R;Takahashi, S;Takeuchi, I
通讯作者：
Takeuchi, I