Increase in the concentration of transforming growth factor beta-1 in bronchoalveolar lavage fluid before development of chronic lung disease of prematurity
Increase in the concentration of transforming growth factor beta-1 in bronchoalveolar lavage fluid before development of chronic lung disease of prematurity
复制标题
DOI：
10.1016/s0022-3476(96)70355-4
复制
发表时间：
1996-04-01
影响因子：
5.1
通讯作者：
Shaw, RJ
中科院分区：
文献类型：
--
作者：
Kotecha, S;Wangoo, A;Shaw, RJ
目的：肺纤维化是慢性早产儿肺疾病(CLD)的显著特征，探讨促纤维化细胞因子转化生长因子β1(1)在慢性早产儿肺疾病发生发展中的作用。方法：对18例早产儿(平均孕周25.7周；结果：慢性阻塞性肺疾病患儿血清中总的转化生长因子-β(1)和活性转化生长因子-β(1)的浓度均高于呼吸窘迫综合征组和对照组。在第4天，当最终患有CLD的婴儿与未进展为CLD的婴儿进行比较时，活性和总的转化生长因子-β(1)的增加最大(活性的转化生长因子-β(1)，39.5比4.6 ng/ml；总的转化生长因子-β(1)，43.8比13.8 ng/ml)。此外，免疫细胞化学研究将PAN-转化生长因子-β定位于从支气管肺泡灌洗液中获得的肺泡巨噬细胞。结论：这些观察表明转化生长因子-β(1)可能参与了慢性肺病患儿肺组织的纤维化反应。
Objective: Pulmonary fibrosis is a prominent feature of chronic lung disease of prematurity (CLD), We sought to determine the influence of the potent profibrotic cytokine transforming growth factor beta-1 (TGF-beta(1)) on the development of CLD.Methods: We determined the concentration of active and total TGF-beta(1) in bronchoalveolar lavage fluid obtained from 18 infants who subsequently had CLD (mean gestation, 25.7 weeks; birth weight, 816 gm), 15 (29.8 weeks, 1493 gm) who recovered from the respiratory distress syndrome, and 7 (35.1 weeks, 2441 gm) control infants.Results: The concentration of both active and total TGF-beta(1) was increased in the infants with CLD when compared with the respiratory distress syndrome and control groups. The increase in active and total TGF-beta(1) was greatest on day 4 of age, when infants who eventually had CLD were compared with those who did not progress to CLD (active TGF-beta(1), 39.5 vs 4.6 ng/ml; total TGF-beta(1), 43.8 vs 13.8 ng/ml). In addition, immunocytochemistry studies localized pan-TGF-beta to alveolar macrophages obtained by bronchoalveolar lavage.Conclusions: These observations indicate that TGF-beta(1) may contribute to the fibrotic response that is observed in the lungs of infants who have CLD.