Transcriptomic uniqueness and commonality of the ion channels and transporters in the four heart chambers.
Transcriptomic uniqueness and commonality of the ion channels and transporters in the four heart chambers.
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DOI：
10.1038/s41598-021-82383-1
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发表时间：
2021-02-02
影响因子：
4.6
通讯作者：
Iacobas DA
中科院分区：
文献类型：
--
作者：
Iacobas S;Amuzescu B;Iacobas DA
用Agilent基因芯片分析了4只成年雄性C57BL/6J小鼠左、右心房和右心室的转录本，以确定四个心腔功能不同的原因。雌性小鼠没有被研究，因为它们的转录组依赖于发情周期阶段。在被量化的16,886个基因中，15.76%的基因在左侧和16.5%的右侧在心房和心脏之间有差异表达，5.8%的基因在两个心房和两个心室之间有差异表达，只有1.2%的基因在两个房间之间有差异表达。这项研究还揭示了基因表达控制和协调方面的内部差异。我们分析了离子通道和转运体，以及心肌收缩、氧化磷酸化、糖酵解/糖异生、钙和肾上腺素能信号通路中的基因。有趣的是，虽然Ank2的表达与左心室所有27个定量结合伙伴都是同步振荡的，但在左、右心房中，Ank2的同相振荡伙伴的比例分别为15%和37%，在右心室中的比例为74%。分析表明，离子通道表达的室间同步性很高，而两个心房之间以及同侧心房和心室之间的同步性明显较低。
Myocardium transcriptomes of left and right atria and ventricles from four adult male C57Bl/6j mice were profiled with Agilent microarrays to identify the differences responsible for the distinct functional roles of the four heart chambers. Female mice were not investigated owing to their transcriptome dependence on the estrous cycle phase. Out of the quantified 16,886 unigenes, 15.76% on the left side and 16.5% on the right side exhibited differential expression between the atrium and the ventricle, while 5.8% of genes were differently expressed between the two atria and only 1.2% between the two ventricles. The study revealed also chamber differences in gene expression control and coordination. We analyzed ion channels and transporters, and genes within the cardiac muscle contraction, oxidative phosphorylation, glycolysis/gluconeogenesis, calcium and adrenergic signaling pathways. Interestingly, while expression of Ank2 oscillates in phase with all 27 quantified binding partners in the left ventricle, the percentage of in-phase oscillating partners of Ank2 is 15% and 37% in the left and right atria and 74% in the right ventricle. The analysis indicated high interventricular synchrony of the ion channels expressions and the substantially lower synchrony between the two atria and between the atrium and the ventricle from the same side.
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影响因子：
5.2
作者：
Iacobas DA;Mgbemena VE;Iacobas S;Menezes KM;Wang H;Saganti PB
通讯作者：
Saganti PB
DOI：
10.1152/ajpheart.00587.2016
发表时间：
2018-01-01
影响因子：
4.8
作者：
Borghetti, Giulia;Eisenberg, Carol A.;Rota, Marcello
通讯作者：
Rota, Marcello
心房颤动和心力衰竭病理生理学中的钙。
影响因子：
4
作者：
Denham NC;Pearman CM;Caldwell JL;Madders GWP;Eisner DA;Trafford AW;Dibb KM
通讯作者：
Dibb KM
影响因子：
16.6
作者：
Anderson, Claire;Khan, Mohsin A. F.;Wong, Frances;Solovieva, Tatiana;Oliveira, Nidia M. M.;Baldock, Richard A.;Tickle, Cheryll;Burt, Dave W.;Stern, Claudio D.
通讯作者：
Stern, Claudio D.
影响因子：
3.4
作者：
Honda, Hisao;Abe, Takaya;Fujimori, Toshihiko
通讯作者：
Fujimori, Toshihiko