Cytokine secretion by γδ and αβ T cells in monophasic experimental autoimmune encephalomyelitis
Cytokine secretion by γδ and αβ T cells in monophasic experimental autoimmune encephalomyelitis
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DOI：
10.1006/jaut.1998.0263
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发表时间：
1999-03-01
影响因子：
12.8
通讯作者：
Arnason, BGW
中科院分区：
文献类型：
--
作者：
Jensen, MA;Dayal, A;Arnason, BGW
在小鼠双相实验性自身免疫性脑脊髓炎（EAE）的过程中，从中枢神经系统（CNS）、淋巴结（LN）、脾脏和血液中分离单核细胞。对单个细胞因子分泌T细胞进行计数。IL-2分泌α β T细胞在疾病发作时在所有部位都大量存在。IL-2分泌γ δ T细胞在整个过程中是罕见的。IFN-γ分泌细胞丰富的所有网站在疾病发作。在疾病发作时，γ δ T细胞占总细胞的7%，占分泌IFN-γ的CNS衍生细胞的20%;在疾病高峰时的值分别为12%和40%。IL-二分泌T细胞在中枢神经系统和LN中是罕见的，并没有从基线值增加脾脏。相比之下，脾脏分泌IL-4的γ δ T细胞在疾病发作时增加到基线值的4倍，在疾病高峰时增加到7倍。从EAE中恢复与IL-2分泌性α β T细胞的全面抑制相关，并且在较小程度上与IFN-γ分泌性α β和γ δ T细胞相关，而IL-4分泌性γ δ T细胞随着疾病的发展而在脾脏中增加。(C)北京：科学出版社.
Mononuclear cells were isolated from the central nervous system (CNS), lymph nodes (LN), spleen and blood, over the course of murine monophasic experimental autoimmune encephalomyelitis (EAE). Individual cytokine secreting T cells were enumerated. IL-2-secreting alpha beta T cells were numerous at all sites at disease onset By disease peak their numbers had fallen profoundly; they remained low thereafter. IL-2 secreting gamma delta T cells were rare throughout. IFN-gamma-secreting cells were plentiful at all sites at disease onset. gamma delta T cells comprised 7% of total and 20% of IFN-gamma-secreting CNS-derived cells at disease onset; values at disease peak were 12 and 40% respectively. IL-di-secreting up T cells were rare in the CNS and LN throughout and did not increase in the spleen from baseline values. In contrast, splenic IL-4-secreting gamma delta T cells had increased to four-fold baseline values at disease onset and seven-fold at disease peak. Recovery from EAE is associated with a global inhibition of IL-2-secreting alpha beta T cells and to a lesser extent with IFN-gamma-secreting alpha beta and gamma delta T cells, whereas IL-4-secreting gamma delta T cells increase in the spleen as disease evolves. (C) 1999 Academic Press.