Metabolomics study on the hepatoprotective effect of scoparone using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry
Metabolomics study on the hepatoprotective effect of scoparone using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry
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DOI：
10.1039/c2an36382h
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Wang, Xijun
中科院分区：
文献类型：
--
作者：
Zhang, Aihua;Sun, Hui;Wang, Xijun
滨蒿内酯是茵陈蒿的重要成分，是我国著名的药用植物，在肝损伤的防治方面显示出广阔的应用前景。然而，肝保护作用的确切分子机制尚未得到全面研究。在这里，代谢组学技术是对生物系统中内源性代谢物的全面评估，并可能提供对机制的额外见解。本研究旨在探讨滨蒿内酯对四氯化碳肝损伤的保护作用及其可能机制。超高效液相色谱/电喷雾电离四重飞行时间质谱（UPLC/ESI-Q-TOF/MS）结合模式识别方法（包括主成分分析（PCA）和偏最小二乘判别分析（PLS-DA））来发现差异代谢物。结果表明，阳性模式中的五种离子是不同的代谢物。功能途径分析表明，这些代谢产物的改变与初级胆汁酸的生物合成、嘧啶代谢有关。值得注意的是，滨蒿内酯具有潜在的药理作用，通过调节多个干扰途径的正常状态。我们的研究结果还表明，强大的代谢组学技术有望获得生物标志物和阐明疾病机制，突出药物发现的见解。
Scoparone is an important constituent of Yinchenhao (Artemisia annua L.), a famous medicinal plant, and displayed bright prospects in the prevention and therapy of liver injury. However, the precise molecular mechanism of hepatoprotective effects has not been comprehensively explored. Here, metabolomics techniques are the comprehensive assessment of endogenous metabolites in a biological system and may provide additional insight into the mechanisms. The present investigation was designed to assess the effects and possible mechanisms of scoparone against carbon tetrachloride-induced liver injury. Ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) combined with pattern recognition approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were integrated to discover differentiating metabolites. Results indicate five ions in the positive mode as differentiating metabolites. Functional pathway analysis revealed that the alterations in these metabolites were associated with primary bile acid biosynthesis, pyrimidine metabolism. Of note, scoparone has a potential pharmacological effect through regulating multiple perturbed pathways to the normal state. Our findings also showed that the robust metabolomics techniques are promising for getting biomarkers and clarifying mechanisms of disease, highlighting insights into drug discovery.