Hydrophobic Liquid-Infused Porous Polymer Surfaces for Antibacterial Applications
Hydrophobic Liquid-Infused Porous Polymer Surfaces for Antibacterial Applications
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DOI：
10.1021/am401532z
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发表时间：
2013-07-24
影响因子：
9.5
通讯作者：
Levkin, Pavel A.
中科院分区：
文献类型：
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作者：
Li, Junsheng;Kleintschek, Tanja;Levkin, Pavel A.
生物膜是环境生物学、水技术、食品卫生以及医疗和技术系统中的一个基本问题。最近推出的光滑液体注入多孔表面（SLIPS）显示出很大的希望，防止生物膜的形成，由于这种表面的低表面能结合其自清洁性能。在这项研究中，我们展示了一种新型的疏水性液体注入的多孔聚（甲基丙烯酸丁酯-共-二甲基丙烯酸乙烯酯）表面（光滑的BMA-EDMA），在BM 2矿物介质中具有抗菌性，在水性环境中具有长期稳定性。我们表明，光滑的BMA-EDMA表面防止生物膜形成的不同菌株的条件致病菌铜绿假单胞菌至少长达7天，在低营养培养基。仅约1.8%的光滑表面被研究中的环境铜绿假单胞菌PA 49菌株覆盖。在未涂覆的玻璃对照中，在相同条件下，表面覆盖率达到55%。然而，在高营养培养基中，更相关的生理条件，生物膜的形成在光滑的表面上被证明是高度依赖于细菌菌株。虽然光滑的表面可以防止大多数测试的铜绿假单胞菌菌株的生物膜形成（类似于1%的表面覆盖率），但是从废水中分离的多重抗性铜绿假单胞菌菌株能够在7天的温育期间覆盖高达12%的表面。对所用铜绿假单胞菌菌株的RAPD-PCR分析表明它们的高基因组变异性，这可能是它们在光滑的BMA-EDMA表面上形成生物膜的差异的原因。结果表明，尽管光滑的BMA-EDMA表面具有很大的抗生物膜形成的潜力，但其抗细菌性能的通用性仍有待提高。
Biofilms represent a fundamental problem in environmental biology, water technology, food hygiene as well as in medical and technical systems. Recently introduced slippery liquid-infused porous surface (SLIPS) showed great promise for preventing biofilm formation owing to the low surface energy of such surface in combination with its self-cleaning properties. In this study we demonstrated a novel hydrophobic liquid-infused porous poly(butyl methacrylate-co-ethylene dimethacrylate) surface (slippery BMA-EDMA) with bacteria-resistance in BM2 mineral medium and long-term stability in aqueous environments. We showed that the slippery BMA-EDMA surface prevents biofilm formation of different strains of opportunistic pathogen Pseudomonas aeruginosa for at least up to 7 days in low nutrient medium. Only similar to 1.8% of the slippery surface was covered by the environmental P. aeruginosa PA49 strain under investigation. In uncoated glass controls the coverage of surfaces reached similar to 55% under the same conditions. However, in high nutrient medium, more relevant to physiological conditions, the biofilm formation on the slippery surface turned out to be highly dependent on the bacterial strain. Although the slippery surface could prevent biofilm formation of most of the P. aeruginosa strains tested (similar to 1% surface coverage), the multiresistant P. aeruginosa strain isolated from wastewater was able to cover up to 12% of the surface during 7 days of incubation. RAPD-PCR analysis of the used P. aeruginosa strains demonstrated their high genome variability, which might be responsible for their difference in biofilm formation on the slippery BMA-EDMA surface. The results show that although the slippery BMA-EDMA surface has a great potential against biofilm formation, the generality of its bacteria resistant properties is still to be improved.