Lectin histochemistry of the rat lymph node:: visualisation of stroma, blood vessels, sinuses, and macrophages.: A contribution to the concept of an immune accessory role of sinus-lining endothelia
Lectin histochemistry of the rat lymph node:: visualisation of stroma, blood vessels, sinuses, and macrophages.: A contribution to the concept of an immune accessory role of sinus-lining endothelia
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DOI：
10.1078/0065-1281-00616
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发表时间：
2002-01-01
影响因子：
2.5
通讯作者：
Schumacher, U
中科院分区：
文献类型：
--
作者：
Düllmann, J;Van Damme, EJM;Schumacher, U
凝集素大Chelidonium majus凝集素（CMA）在大鼠脾白髓所有血管和内层窦、基质网状网（RM）和淋巴滤泡树突状细胞中的内皮可见。本研究的目的是分析CMA和其他一些凝集素对大鼠RIM、血管结构和淋巴结巨噬细胞的标记作用，发现CMA可以对整个RM、树突状细胞、鼻窦衬细胞和所有类型的血管进行染色。淋巴结窦壁细胞用CMA和甘露糖、GalNac和唾液酸特异性凝集素标记。此外，淋巴结巨噬细胞首先被甘露糖特异性凝集素标记。在大鼠脾脏中未观察到广泛的鼻窦凝集素结合模式-鼻窦内壁细胞和树突状细胞的cma反应性证实了淋巴结鼻窦内壁内皮是前体或特殊类型的免疫辅助细胞的假设。
The lectin Chelidonium majus agglutinin (CMA) was previously shown to visualise endothelia of all blood vessels and those lining sinuses of red pulp, stromal reticular meshwok (RM) and dendritic cells of lymphatic follicles in white pulp of the spleen in rats. The aim of the present study was the analysis of CMA and some other lectins in labelling RIM, vascular structures and macrophages in lymph nodes of rats, It appeared that CMA stained the entire RM, dendritic cells, lining cells of sinuses and all types of blood vessels. Sinus-lining cells of lymph nodes were labelled with CMA and mannose-, GalNac-, and sialic acid-specific lectins. Moreover, lymph node macrophages were labelled above all by mannose specific lectins. The broad lectin-binding pattern of sinuses not observed in rat spleen- and CMA-reactivity of both sinus-lining and dendritic cells corroborates the hypothesis that lymph node sinus-lining endothelia are precursors or a special type of immune accessory cells.