MMP inhibition prevents human lens epithelial cell migration and contraction of the lens capsule
MMP inhibition prevents human lens epithelial cell migration and contraction of the lens capsule
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DOI：
10.1136/bjo.2003.034629
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发表时间：
2004-07-01
影响因子：
4.1
通讯作者：
Khaw, PT
中科院分区：
文献类型：
--
作者：
Wong, TTL;Daniels, JT;Khaw, PT
目的：白内障术后后囊膜收缩是由残留晶状体上皮细胞的活动引起的。基质金属蛋白酶（MMPs）是一组蛋白水解酶，在伤口愈合后的细胞迁移和细胞介导的收缩中起重要作用。作者研究了抑制MMP活性是否可以减少晶状体上皮细胞的迁移，从而导致细胞介导的囊收缩减少。方法：培养人供体晶状体囊，并用广谱MMP抑制剂Ilomastat （GM6001）处理。ELISA法检测MMP-2和MMP-9的产生。数字测量细胞向后囊膜的迁移和后囊膜的收缩。结果：MMP抑制显著减少晶状体上皮细胞向后囊膜的迁移（p < 0.05），减少后囊膜收缩（p < 0.05）。结论：依洛司他可显著减少晶状体上皮细胞向后囊膜表面的迁移，抑制后囊膜收缩。抑制MMP可能在后囊膜混浊的治疗中起作用。
Purpose: The development of posterior capsule contraction following cataract surgery is caused by the activity of residual lens epithelial cells. Matrix metalloproteinases (MMPs) are a group of proteolytic enzymes, which are essential for cell migration and cell mediated contraction following wound healing. The authors investigated whether inhibiting MMP activity can reduce lens epithelial cell migration and as a result, lead to a reduction in cell mediated capsule contraction.Methods: Human donor lens capsules were cultured and treated with a broad spectrum MMP inhibitor, Ilomastat (GM6001). MMP-2 and MMP-9 production were determined by ELISA. Cell migration onto the posterior capsule and capsule contraction were digitally measured.Results: MMP inhibition significantly reduced lens epithelial cell migration onto the posterior capsule (p < 0.05), and a reduction in capsule contraction was observed (p < 0.05).Conclusions: Ilomastat significantly reduced lens epithelial cell migration onto the posterior capsule surface and inhibited capsule contraction. MMP inhibition may have a role in the therapeutic treatment of posterior capsule opacification.