STRUCTURE OF A BACILLUS-SUBTILIS BACTERIOPHAGE SPO1 GENE ENCODING A RNA-POLYMERASE SIGMA-FACTOR
STRUCTURE OF A BACILLUS-SUBTILIS BACTERIOPHAGE SPO1 GENE ENCODING A RNA-POLYMERASE SIGMA-FACTOR
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DOI：
10.1073/pnas.80.5.1236
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
PERO, J
中科院分区：
文献类型：
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作者：
COSTANZO, M;PERO, J
B的基因28。枯草噬菌体SPO 1编码调节蛋白，a σ。已知为σ gp 28的因子，其与细菌核心RNA聚合酶结合以指导噬菌体中间基因启动子的识别，中间启动子在以λ为中心的2个区域中显示独特且保守的核苷酸序列。mRNA合成起始点上游10和35个碱基对。本文报道了28号基因的克隆及其全序列。σ gp 28是220个氨基酸的25，707-道尔顿蛋白质。基因28的核苷酸序列和推导的σ gp 28的氨基酸序列都没有表现出与大肠杆菌σ的基因或蛋白质序列的广泛同源性。因子
Gene 28 of B. subtilis bacteriophage SPO1 codes for a regulatory protein, a .sigma. factor known as .sigma.gp28, that binds to the bacterial core RNA polymerase to direct the recognition of phage middle gene promoters, middle promoters exhibit distinctive and conserved nucleotide sequences in 2 regions centered .apprx. 10 and 35 base pairs upstream from the start point of mRNA synthesis. The cloning of gene 28 and its complete nucleotide sequence are reported. .sigma.gp28 is a 25,707-dalton protein of 220 amino acids. Neither the nucleotide sequence of gene 28 nor the inferred amino acid sequence of .sigma.gp28 exhibits extensive homology to the gene or protein sequence of Escherichia coli .sigma. factor.