ORGANIZATION OF THE HUMAN CHOLESTERYL ESTER TRANSFER PROTEIN GENE
ORGANIZATION OF THE HUMAN CHOLESTERYL ESTER TRANSFER PROTEIN GENE
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DOI：
10.1021/bi00458a004
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发表时间：
1990-02-13
期刊：
影响因子：
2.9
通讯作者：
TALL, AR
中科院分区：
文献类型：
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作者：
AGELLON, LB;QUINET, EM;TALL, AR
血浆胆固醇酯转移蛋白（CETP）催化磷脂和中性脂在脂蛋白之间的转移。因此，该蛋白可能在调节血浆脂蛋白水平中起重要作用。我们已经确定了人类CETP基因的初级结构和组织。细胞DNA的Southern印迹表明每个单倍体基因组存在单个CETP基因拷贝。对三个重叠的基因组克隆的分析表明，该基因全长约25 kbp，包含16个外显子（大小范围为32-250 bp）。总的来说，CETP基因的序列和组织不像其他脂质代谢酶或载脂蛋白。然而，将CETP序列与序列数据库中的序列进行一次一个外显子的比较，发现在人类CETP、载脂蛋白a - iv和a - i以及脂蛋白脂肪酶的信号序列疏水核心内的五肽序列（ValLeuThrLeuAla）具有惊人的一致性。该五肽序列在其他蛋白质的信号序列中未发现，提示其可能介导与脂质代谢或转运相关的特殊功能。
The plasma cholesteryl ester transfer protein (CETP) catalyzes the transfer of phospholipids and neutral lipids between the lipoproteins. Thus, this protein may be important in modulating lipoprotein levels in the plasma. We have determined the primary structure and organizatin of the human CETP gene. Southern blotting of cellular DNA indicating a single copy of the CETP gene exists per haploid genome. Analysis of three overlapping genomic clones showed that the gene spans approximately 25 kbp and contains 16 exons (size range 32-250 bp). Overall, the sequence and organization of the CETP gene do not resemble those of other lipid-metabolizing enzymes or apolipoproteins. However, comparison of the CETP sequence, one exon at a time, with the sequences in the sequence databases revealed a striking identity of a pentapeptide sequence (ValLeuThrLeuAla) within the hydrophobic core of the signal sequences of human CETP, apolipoproteins A-IV and A-I, and lipoprotein lipase. This pentapeptide sequence was not found in the signal sequences of other proteins, suggesting that it may mediate a specialized function related to lipid metabolism or transport.