Shear stress promotes differentiation of stem cells from human exfoliated deciduous teeth into endothelial cells via the downstream pathway of VEGF-Notch signaling.
Shear stress promotes differentiation of stem cells from human exfoliated deciduous teeth into endothelial cells via the downstream pathway of VEGF-Notch signaling.
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剪切应力通过 VEGF-Notch 信号下游途径促进人脱落乳牙干细胞分化为内皮细胞
DOI：
10.3892/ijmm.2018.3761
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发表时间：
2018-10
影响因子：
5.4
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Wang P;Zhu S;Yuan C;Wang L;Xu J;Liu Z
本文研究了剪切力对人乳牙脱落干细胞向内皮细胞分化的影响。剪切应力处理SHED后，采用逆转录-定量聚合酶链反应（RT-qPCR）分析动脉标志物的mRNA表达，采用蛋白质印迹分析血管生成标志物的蛋白表达。此外，进行体外基质胶血管生成测定以评价血管样结构形成。还使用相应的ELISA试剂盒定量剪切应力后SHED的血管内皮生长因子（VEGF）的分泌蛋白表达水平。接种在Matrigel上的未经处理的SHED在任何时间点都不能形成血管样结构，而用剪切应力处理的组在4、8和12 h形成了一些血管样结构。当用EphrinB 2-siRNA处理SHED 24时，血管样结构形成的能力被抑制。切应力处理后，VEGF、VEGFR 2、DLL 4、Notch 1、EphrinB 2、Hey 1和Hey 2（动脉标志物）基因表达显著上调，VEGFR 2、EphrinB 2、CD 31、Notch 1、DLL 4、Hey 1和Hey 2蛋白水平也显著上调。静脉标志物EphB 4的mRNA和蛋白表达水平均下调。切应力处理组分泌的上清液中VEGF蛋白的平均浓度显著增加。总之，切应力能够诱导人脱落乳牙干细胞向动脉内皮细胞分化，VEGF-DLL 4/Notch-EphrinB 2信号通路参与了这一过程。
Effects of shear stress on endotheliaxl differentiation of stem cells from human exfoliated deciduous teeth (SHEDs) were investigated. SHEDs were treated with shear stress, then reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to analyse the mRNA expression of arterial markers and western blot analysis was performed to analyse protein expression of angiogenic markers. Additionally, in vitro matrigel angiogenesis assay was performed to evaluate vascular-like structure formation. The secreted protein expression levels of the vascular endothelial growth factor (VEGF) of SHEDs after shear stress was also quantified using corresponding ELISA kits. Untreated SHEDs seeded on Matrigel cannot form vessel-like structures at any time points, whereas groups treated with shear stress formed a few vessel-like structures at 4, 8 and 12 h. When SHEDs were treated with EphrinB2-siRNA for 24, the capability of vessel-like structure formation was suppressed. After being treated with shear stress, the expression of VEGF, VEGFR2, DLL4, Notch1, EphrinB2, Hey1 and Hey2 (arterial markers) gene expression was significantly upregulated, moreover, the protein levels of VEGFR2, EphrinB2, CD31, Notch1, DLL4, Hey1, and Hey2 were also significantly upregulated. Both the mRNA and protein expression levels of EphB4 (venous marker) were downregulated. The average VEGF protein concentration in supernatants secreted by shear stress treated SHEDs groups increased significantly. In conclusion, shear stress was able to induce arterial endothelial differentiation of stem cells from human exfoliated deciduous teeth, and VEGF-DLL4/Notch-EphrinB2 signaling was involved in this process.
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影响因子：
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作者：
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通讯作者：
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