Demonstration of a PDMS-based bio-microactuator using cultured cardiomyocytes to drive polymer micropillars
Demonstration of a PDMS-based bio-microactuator using cultured cardiomyocytes to drive polymer micropillars
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DOI：
10.1039/b512099c
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发表时间：
2006-02-01
期刊：
影响因子：
6.1
通讯作者：
Kitamori, T
中科院分区：
文献类型：
--
作者：
Tanaka, Y;Morishima, K;Kitamori, T
天然细胞功能越来越多地用于集成化学系统，如生化反应器和生物传感器。我们建议利用心肌细胞固有的机械功能，将化学能转化为机械能。在这份报告中，我们证明了我们提出的聚（二甲基硅氧烷）（PDMS）为基础的心肌细胞生物微驱动器的工作原理，使用制造的PDMS微柱驱动附着的脉动心肌细胞的重复运动。制造并修饰了嵌入微柱阵列的PDMS片材，用于培养中的心肌细胞附着。原代乳鼠心肌细胞在微阵列上培养，成功贴附于微柱和基质上，表现出典型的自发搏动表型。微柱与偶联的细胞自发地跳动，没有任何触发。拍频在37摄氏度时为1.4赫兹，在我们的观察中，拍得最强烈的柱子顶部的位移为2.8 +/- 0.2 μ m。根据该结果，估计培养的心肌细胞的收缩力超过3.5 μ N。估计的力远远大于先前描述的基于水凝胶的心肌细胞生物微致动器（K. Morishima等人，在Micro Total Analysis Systems 2003，ed.M. A. Northrup等人，传感器研究基金会，San Diego，CA，第2卷，pp. 1125-1128）。使用培养的心肌细胞作为生物微致动器设计的基础材料的PDMS兼容性被验证。这种基于PDMS的细胞微致动器在不更换培养基的情况下工作了大约一周，并且该系统可以在未来开发用于各种目的，作为自致动和高效的机械化学换能器，而无需外部能源。
Natural cellular functions are increasingly exploited for integrated chemical systems such as biochemical reactors and biosensors. We propose to utilize the intrinsic mechanical function of cardiomyocytes, converting chemical energy into mechanical energy. In this report, we demonstrate the working principle of our proposed poly(dimethylsiloxane) (PDMS) based cardiomyocyte bio-microactuator using fabricated PDMS micropillars driven to repetitive motion by attached pulsating cardiomyocytes. Sheets of PDMS embedded with an array of micropillars were fabricated and modified for cardiomyocyte attachment in culture. Primary neonatal rat cardiomyocytes were cultured on the array, attaching to the micropillars and substratum successfully, and exhibiting their typical spontaneous, pulsatile phenotype. Micropillars beat with the coupled cells spontaneously without any triggers. The beat frequency was 1.4 Hz at 37 degrees C and the displacement of the top of the pillar that beat most strongly in our observation was 2.8 +/- 0.2 mu m. From this result, contractile forces of cultured cardiomyocytes were estimated to exceed 3.5 mu N. The estimated force is far greater than that of a previously described hydrogel-based cardiomyocyte bio-microactuator (K. Morishima et al., in Micro Total Analysis Systems 2003, ed. M. A. Northrup et al., The Transducers Research Foundation, San Diego, CA, vol. 2, pp. 1125-1128). PDMS compatibility as a base material for bio-microactuator design using cultured cardiomyocytes was verified. This PDMS-based cell microactuator worked for about one week without exchange of the culture medium, and this system could be developed for various purposes in the future as self-actuated and efficient mechanochemical transducers without external energy source requirements.