PERMEATION OF GLYCEROL AND PROPANE-1,2-DIOL INTO HUMAN PLATELETS
PERMEATION OF GLYCEROL AND PROPANE-1,2-DIOL INTO HUMAN PLATELETS
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DOI：
10.1016/0011-2240(90)90002-l
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发表时间：
1990-04-01
期刊：
影响因子：
2.7
通讯作者：
PEGG, DE
中科院分区：
文献类型：
--
作者：
ARNAUD, FG;PEGG, DE
人血小板对甘油（GLY）和丙烷-1,2-二醇（丙二醇，PG）的渗透性是通过测量它们在这些溶质溶液中突然浸入后体积变化的时间过程来确定的。介绍了一种简单的光散射法，并对其进行了校正，以测量血小板平均体积。数据通过Kedem-Katchalsky （K-K）方程进行分析，该方程经过修改以考虑细胞内和细胞外溶质的非理想行为。K-K参数在2、21、37度处的值。C分别为：水力导率（Lp）为1.1倍。10-7, 7。10-7和3倍。10 - 6 cm.cntdot.sec - 1. - cntdot.atm - 1;PG （.omega. rtpg）溶质渗透率为1.9倍。10-6, 2.8倍。10-5，和1.3倍。身手cm.cntdot.sec-1尤其;GLY的溶质渗透率。RTGLY)， 21度和37度。只有C，是2.6倍。10-7和1.4倍。10 - 6 cm.cntdot.sec-1。反射系数（.sigma.）始终为1。相关活化能为-Lp， 16.5 kcal.cntdot.mol-1；.omega。RTPG, 20.5 kcal.cntdot.mol-1；和.omega。RTGLY 17.9 kcal.cntdot.mol-1。这些数据的使用是通过计算添加和去除GLY和PG的时间表来说明的，这样血小板体积的变化幅度就保持在预定的范围内。
The permeability of human platelets to glycerol (GLY) and propane-1,2-diol (propylene glycol, PG) has been determined by measuring the time course of their change in volume following abrupt immersion in solutions of these solutes. A simple light-scattering method, and its calibration to measure mean platelet volume is described. The data are analyzed by means of the Kedem-Katchalsky (K-K) equations, modified to take into account the nonideal behavior of both intracellular and extracellular solutes. The values of the K-K parameters at 2, 21, and 37.degree. C, respectively, were as follows: the hydraulic conductivities (Lp) were 1 .times. 10-7, 7 .times. 10-7 and 3 .times. 10-6 cm.cntdot.sec-1.cntdot.atm-1; the solute permeabilities for PG (.omega.RTPG) were 1.9 .times. 10-6, 2.8 .times. 10-5, and 1.3 .times. 10-4 cm.cntdot.sec-1; the solute permeabilities for GLY (.omega.RTGLY), at 21 and 37.degree. C only, were 2.6 .times. 10-7 and 1.4 .times. 10-6 cm.cntdot.sec-1. The reflection coefficient (.sigma.) was 1 throughout. The relevant activation energies were -Lp, 16.5 kcal.cntdot.mol-1; .omega.RTPG, 20.5 kcal.cntdot.mol-1; and .omega.RTGLY, 17.9 kcal.cntdot.mol-1. The use of these data is illustrated by computing schedules for the addition and removal of GLY and PG so that the amplitudes of changes in platelet volume are held within predetermined limits.