Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles.
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles.
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具有用于粒子空间跟踪的多重电子检测的微流体平台。
DOI：
10.3791/55311
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
A. F. Sarioglu
中科院分区：
文献类型：
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作者：
Ningquan Wang;Ruxiu Liu;A. F. Sarioglu
生物样品的微流体处理通常涉及在各种力场下对悬浮颗粒的差分操纵，以便基于感兴趣的生物特性在空间上使样品破碎。为了将所得的空间分布用作测定读出，微流体装置通常经受显微镜分析，这需要具有较高成本和降低的便携性的复杂仪器。为了解决这一限制，我们已经开发了一种集成的电子传感技术，用于在微流控芯片上的不同位置处对颗粒进行多重检测。我们的技术，称为微流体编码，结合了电阻脉冲传感与码分多址，将2D空间信息压缩成1D电信号。在本文中，我们提出了一个实际的演示的微流控CODES技术检测和大小分布在多个微流控通道培养的癌细胞。正如高速显微镜所验证的那样，我们的技术可以准确地分析致密的细胞群，而不需要外部仪器。因此，微流体CODES可以潜在地实现低成本的集成芯片实验室设备，其非常适合于生物样品的即时检测。
Microfluidic processing of biological samples typically involves differential manipulations of suspended particles under various force fields in order to spatially fractionate the sample based on a biological property of interest. For the resultant spatial distribution to be used as the assay readout, microfluidic devices are often subjected to microscopic analysis requiring complex instrumentation with higher cost and reduced portability. To address this limitation, we have developed an integrated electronic sensing technology for multiplexed detection of particles at different locations on a microfluidic chip. Our technology, called Microfluidic CODES, combines Resistive Pulse Sensing with Code Division Multiple Access to compress 2D spatial information into a 1D electrical signal. In this paper, we present a practical demonstration of the Microfluidic CODES technology to detect and size cultured cancer cells distributed over multiple microfluidic channels. As validated by the high-speed microscopy, our technology can accurately analyze dense cell populations all electronically without the need for an external instrument. As such, the Microfluidic CODES can potentially enable low-cost integrated lab-on-a-chip devices that are well suited for the point-of-care testing of biological samples.