Fluorescence analysis of micro-scale surface modification using ultrafine capillary atmospheric pressure plasma jet for biochip fabrication
Fluorescence analysis of micro-scale surface modification using ultrafine capillary atmospheric pressure plasma jet for biochip fabrication
复制标题
使用超细毛细管大气压等离子体射流进行生物芯片制造的微尺度表面修饰的荧光分析
DOI：
10.1007/978-3-319-46490-9_34
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and Tomy Abuzairi
中科院分区：
文献类型：
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作者：
Masaaki Nagatsu;Masahiro Kinpara;and Tomy Abuzairi
在这项研究中，我们提出了微尺度的表面功能化技术，使用无掩模，多功能，简单的工具，由纳米或微毛细管大气压等离子体射流（APPJs）为代表。利用荧光技术研究了氨基或羧基在聚合物基底上尺寸可控的表面功能化。此外，我们证明了功能化的微尺度图案上的生物分子的成功连接，表明使用超细毛细管APPJ作为生物传感，组织工程和相关生物医学应用的通用工具的可能性。利用这种技术，我们研究了通过超细APPJ将生物分子图案化到CNT点阵列上用于生物芯片的制作。利用具有微毛细管的超细APPJ在指定的CNT点上官能化氨基。通过两阶段的等离子体处理，预处理和后处理，进行超细APPJ功能化CNT阵列。利用生物素-亲和素体系等生物分子体系对碳纳米管固定生物分子的可行性进行了研究。通过将生物分子图案化到CNT微阵列上，成功地证明了这种技术用于微生物芯片应用的可能性。
In this study, we propose the micro-scale surface functionalization techniques using a maskless, versatile, simple tool, represented by a nano- or micro-capillary atmospheric pressure plasma jet (APPJs). We show the possibility of size-controlled surface functionalization on polymer substrate with amino groups or carboxyl groups by using fluorescent technique. Moreover, we prove the successful connection of biomolecules on the functionalized micro-scale patterns, indicating the possibility to use ultrafine capillary APPJs as versatile tools for biosensing, tissue engineering, and related biomedical applications. With use of this technology, we study the biomolecules patterning onto CNT dot array via ultrafine APPJ for biochip fabrication. An ultrafine APPJ with a micro-capillary was utilized to functionalize amino groups on designated CNT spots. Two-stage plasma treatments, pre-treatment and post-treatment, were conducted to functionalize CNT array by ultrafine APPJ. Biomolecules system, such as biotin-avidin system was employed to assess the feasibility of biomolecule immobilization on CNT. The possibility of this technique for micro-biochip applications was successfully demonstrated by patterning biomolecules onto CNT microarrays.