Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4.
Multiple effects of Escherichia coli Nissle 1917 on growth, biofilm formation, and inflammation cytokines profile of Clostridium perfringens type A strain CP4.
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DOI：
10.1111/2049-632x.12153
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发表时间：
2014-04
影响因子：
3.3
通讯作者：
Curtiss R 3rd
中科院分区：
文献类型：
--
作者：
Jiang Y;Kong Q;Roland KL;Wolf A;Curtiss R 3rd
产气荚膜梭菌是引起食物中毒、坏死性肠炎、气性坏疽甚至死亡的重要革兰氏阳性病原体。大肠杆菌Nissle 1917（EcN）是一种具有良好特征的益生菌菌株，具有已证明的益处。在这项研究中，我们评估了EcN对C.产气荚膜杆菌体外共培养实验表明，EcN抑制了C.以剂量依赖性方式抑制产气荚膜杆菌。C.将产气荚膜杆菌与EcN无细胞上清液（CFSE）孵育，表明生长抑制是由共孵育期间的营养竞争引起的。体外研究表明，用EcN预孵育不抑制C。产气荚膜梭菌对Caco-2细胞的粘附，但确实减少了C. 24 h后产气荚膜杆菌总数、毒素产生和细胞毒性。在C.产气荚膜杆菌生物膜。最后，将EcN与RAW 264.7细胞预孵育显著降低了由引入C.产气荚膜杆菌我们的研究结果表明，EcN可以抑制C.体外条件下的产气荚膜杆菌。
Clostridium perfringens is an important Gram-positive pathogen responsible for food poisoning, necrotic enteritis, gas gangrene, and even death. Escherichia coli Nissle 1917 (EcN) is a well-characterized probiotic strain with demonstrated benefits. In this study we evaluated the effects of EcN on growth, toxin production, biofilm formation and inflammatory cytokine responses of C. perfringens. In vitro co-culture experiments demonstrated that EcN inhibited growth, gas production and toxin production (α-toxin and NetB) of C. perfringens in a dose dependent manner. The growth inhibition effect was not observed when C. perfringens was incubated with EcN cell free supernatants (CFSE), suggesting that growth inhibition was caused by nutrition competition during co-incubation. In vitro studies demonstrated that pre-incubation with EcN did not inhibit C. perfringens attachment to Caco-2 cells, but did reduce C. perfringens total number, toxin production and cytotoxicity after 24 h. The similar growth inhibition results were also observed during the formation of C. perfringens biofilm. Finally, pre-incubation of EcN with RAW264.7 cells significantly decreased the production of inflammatory cytokines caused by introduction of C. perfringens. Our results indicate that EcN can inhibit many of the pathological effects of C. perfringens in vitro conditions.