Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis
Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis
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DOI：
10.1007/s10577-016-9518-8
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发表时间：
2016-05-01
影响因子：
2.6
通讯作者：
Houben, Andreas
中科院分区：
文献类型：
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作者：
Aliyeva-Schnorr, Lala;Stein, Nils;Houben, Andreas
作物野生近缘种被认为是驯化作物等位基因多样性的重要遗传资源。然而，由于杂交障碍和基因组不相容，它们在育种计划中的利用往往受到限制。大麦的野生近缘种具有吸引人的特性，因此等位基因多样性使大麦能够更好地适应不断变化的环境条件。因此，更好地了解栽培大麦与野生近缘大麦的基因组同源性是一项重要的任务。利用荧光原位杂交（FISH）技术，将22个基因组单拷贝探针和14个互补DNA （cDNA）染色体3h特异性探针定位到黄貂草（Hordeum bulbosum）、海洋貂草（Hordeum marinum）、耻骨貂草（Hordeum pubbiflorum）、鼠貂草（Hordeum murinum）和谷物貂（Secale cereale）的染色体上，以观察近缘种基因组共线性。大多数探针显示了可靠的信号，证实了栽培大麦与相关品种之间的同源性。FISH标记的顺序和位置的差异表明了Hordeum属内的序列移动或小规模的染色体重排，并证实了大麦和黑麦之间的染色体重排。无重复基因组探针和cDNA探针的比较表明，含有基因的单拷贝基因组DNA （gDNA）探针在基于fish的序列分析中表现得更可靠。
Crop wild relatives are considered as important genetic resources of allelic diversity for domesticated crop species. Their utilization in breeding programs, however, is often limited due to crossing barriers and genome incompatibilities. Wild relatives of barley possess attractive properties and hence allelic diversity for adapting barley better to changing environmental conditions. Therefore, gaining a better knowledge about genomic synteny between cultivated barley and wild relatives of the same genus is an important task. To visualize genomic collinearity in related species, 22 genomic single-copy and 14 complementary DNA (cDNA) chromosome 3H-specific probes were mapped to the chromosomes of Hordeum bulbosum, Hordeum marinum, Hordeum pubiflorum, Hordeum murinum, and Secale cereale by fluorescent in situ hybridization (FISH). Most probes showed reliable signals confirming homoeology between cultivated barley and related species. Differences in order and position of FISH markers demonstrated sequence movements or small-scale chromosomal rearrangements within genus Hordeum and confirmed interchromosomal rearrangements between barley and rye. Comparison between repeat-free genomic and cDNA probes showed that gene-containing single-copy genomic DNA (gDNA) probes are performing more reliably for FISH-based analysis of synteny.