Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1 infected individuals on prolonged effective antiretroviral therapy
Use of real-time PCR and molecular beacons to detect virus replication in human immunodeficiency virus type 1 infected individuals on prolonged effective antiretroviral therapy
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DOI：
10.1128/jvi.73.7.6099-6103.1999
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Markowitz, M
中科院分区：
文献类型：
--
作者：
Lewin, SR;Vesanen, M;Markowitz, M
我们设计了一种新颖、精确且灵敏的检测方法，通过使用实时 PCR 和分子信标来测量 HIV-1 感染者外周血单核细胞中未剪接的 (US) 人类免疫缺陷病毒 1 型 (HIV-1) mRNA。根据血浆 HIV-1 RNA 的纵向测量，个体被分为良好抑制 (WS) 或部分抑制。在 WS 个体中，随着时间的推移，US mRNA 无法检测到的个体比例明显更高；然而，经过 24 个月的有效抗病毒治疗后，30% 的 WS 受试者仍可检测到 US mRNA。
We have designed a novel, precise, and sensitive assay to measure unspliced (US) human immunodeficiency virus type 1 (HIV-1) mRNA in peripheral blood mononuclear cells of HIV-1-infected individuals by using real-time PCR and molecular beacons. Individuals were classified as either well suppressed (WS) or partially suppressed, based on longitudinal measurements of plasma HIV-1 RNA. The proportion of individuals with US mRNA undetectable over time was significantly higher among WS individuals; however, 30% of WS subjects still had detectable US mRNA after 24 months of effective antiviral therapy.