Identification and characterization of OsEBS, a gene involved in enhanced plant biomass and spikelet number in rice
Identification and characterization of OsEBS, a gene involved in enhanced plant biomass and spikelet number in rice
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OsEBS 的鉴定和表征，OsEBS 是一种与增强水稻植物生物量和小穗数量有关的基因。
DOI：
10.1111/pbi.12097
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发表时间：
2013-12-01
影响因子：
13.8
通讯作者：
Luo, Xiaojin
中科院分区：
文献类型：
--
作者：
Dong, Xianxin;Wang, Xiaoyan;Luo, Xiaojin
普通野生稻(Oryza rufipogon Griff.)是水稻改良的重要基因库。以水稻品种贵超2号为背景，利用一组普通野生稻的导入系，研究了一个与株高、叶片大小和穗型相关的数量性状基因座qGP5-1。我们克隆和鉴定了qGP5-1，并证实了新发现的基因OsEBS(提高生物量和小穗数)增加了株高、叶片大小和每穗小穗数，从而提高了单株的籽粒总产量。我们的结果表明，OsEBS表达植株营养器官大小的增加在很大程度上是由细胞数量的增加引起的。序列比对表明，OsEBS蛋白含有一个与Hsp70 N端保守的ATPase结构域高度相似的区域，但它缺少多肽结合区的C末端区域和C末端的LID。更多的结果表明，OsEBS基因在本研究中不具有典型的Hsp70特征。此外，转OsEBS基因的拟南芥表现出与转OsEBS基因水稻相似的表型，表明OsEBS在植物中具有保守的功能。我们通过图位克隆的方法克隆和鉴定了一个控制水稻生物量和小穗数的新的QTL OsEBS，该基因可能在水稻产量改良中发挥作用。
Common wild rice (Oryza rufipogon Griff.) is an important genetic reservoir for rice improvement. We investigated a quantitative trait locus (QTL), qGP5-1, which is related to plant height, leaf size and panicle architecture, using a set of introgression lines of O. rufipogon in the background of the Indica cultivar Guichao2 (Oryza sativa L.). We cloned and characterized qGP5-1 and confirmed that the newly identified gene OsEBS (enhancing biomass and spikelet number) increased plant height, leaf size and spikelet number per panicle, leading to an increase in total grain yield per plant. Our results showed that the increased size of vegetative organs in OsEBS-expressed plants was enormously caused by increasing cell number. Sequence alignment showed that OsEBS protein contains a region with high similarity to the N-terminal conserved ATPase domain of Hsp70, but it lacks the C-terminal regions of the peptide-binding domain and the C-terminal lid. More results indicated that OsEBS gene did not have typical characteristics of Hsp70 in this study. Furthermore, Arabidopsis (Arabidopsis thaliana) transformed with OsEBS showed a similar phenotype to OsEBS-transgenic rice, indicating a conserved function of OsEBS among plant species. Together, we report the cloning and characterization of OsEBS, a new QTL that controls rice biomass and spikelet number, through map-based cloning, and it may have utility in improving grain yield in rice.