A purification step prior to commercial sensitive immunoassay is necessary to achieve clinical usefulness when quantifying serum 17β-estradiol in prepubertal children
A purification step prior to commercial sensitive immunoassay is necessary to achieve clinical usefulness when quantifying serum 17β-estradiol in prepubertal children
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DOI：
10.1530/eje-07-0403
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发表时间：
2008-01-01
影响因子：
5.8
通讯作者：
Norjavaara, Ensio
中科院分区：
文献类型：
--
作者：
Ankarberg-Lindgren, Carina;Norjavaara, Ensio
目的：为了测试的临床实用性的敏感的商业免疫测定测定低17 β-雌二醇浓度在children.Methods：三个商业雌二醇免疫测定的检测下限和临床实用性（功能灵敏度）进行了验证，使用500血清从青春期前和青春期的儿童和55个汇集的血清。三种免疫测定法由两种改良的直接免疫测定法组成;一种RIA（Spectria Escherichia RIA）和一种时间分辨荧光免疫测定法（AutoDELFIA Escherichia）。两者都具有与抗体浓度相关的增加的血清体积和延长的孵育时间。第三种方法是用乙醚提取纯化浓缩血清，然后用改良的SpectriaEscheriaRIA测定。结果：直接放免法的检测下限为9 pmol/l，临床实用性为30 pmol/l。提取RIA测定AutoDELFIA为11和50 pmol/l，血清为4和6 pmol/l。当测量来自乳房1-2期女孩的血清池时，与提取RIA相比，直接RIA和AutoDELFIA导致17 β-雌二醇浓度显着更高（+58和+267 ' %，P
Objective: To test the clinical usefulness of sensitive commercial immunoassays for determination of low 17 beta-estradiol concentrations in children.Methods: The lower limit of detection and clinical usefulness (functional sensitivity) of three commercial estradiol immunoassays were validated by use of 500 sera from prepubertal and pubertal children and 55 pooled sera. The three immunoassays consisted of two modified direct immunoassays; one RIA (Spectria Estradiol RIA) and one time-resolved fluoroimmunoassay (AutoDELFIA Estradiol). both with increased serum volume in relation to antibody concentration and extended incubation time. In the third method, serum was purified and concentrated using diethyl ether extraction prior to measurement by the modified Spectria Estradiol RIA.Results: The lower limits of detection and clinical usefulness were 9 and 30 pmol/l for the direct RIA. 11 and 50 pmol/l for the AutoDELFIA, and 4 and 6 pmol/l for serum determined by extraction RIA. When measuring the serum pool originating from girls at breast stages 1-2, the direct RIA and AutoDELFIA resulted in significantly higher 17 beta-estradiol concentrations when compared with the extraction RIA (+58 and +267'%, P