Towards understanding the catalytic core structure of the spliceosome
Towards understanding the catalytic core structure of the spliceosome
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DOI：
10.1042/bst0330447
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发表时间：
2005-06-01
影响因子：
3.9
通讯作者：
Brow, DA
中科院分区：
文献类型：
--
作者：
Butcher, SE;Brow, DA
剪接体催化真核生物核前体mRNA（前体mRNA）的剪接。前体mRNA剪接对于去除前体mRNA的内部非编码区（内含子）并在翻译前将剩余片段（外显子）连接到mRNA中至关重要。剪接体是五种RNA（U1，U2，U4，U5和U6）和数十种相关蛋白质的复杂组装体。虽然剪接体的高分辨率结构还没有，但对它的结构和功能的理解已经取得了进展。越来越多的证据表明，U2和U6 RNA，这五个，可能有助于催化前mRNA剪接。本文就U2和U6 RNA的结构和功能的研究进展作一综述。
The spliceosome catalyses the splicing of nuclear pre-mRNA (precursor mRNA) in eukaryotes. Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The spliceosome is a complex assembly of five RNAs (U1, U2, U4, U5 and U6) and many dozens of associated proteins. Although a high-resolution structure of the spliceosome is not yet available, inroads have been made towards understanding its structure and function. There is growing evidence suggesting that U2 and U6 RNAs, of the five, may contribute to the catalysis of pre-mRNA splicing. In this review, recent progress towards understanding the structure and function of U2 and U6 RNAs is summarized.