Effects of exogenous short-chain N-acyl homoserine lactone on denitrifying process of Paracoccus denitrificans
Effects of exogenous short-chain N-acyl homoserine lactone on denitrifying process of Paracoccus denitrificans
复制标题
外源短链N-酰基高丝氨酸内酯对反硝化副球菌反硝化过程的影响
DOI：
10.1016/j.jes.2016.05.019
复制
发表时间：
2017-04-01
影响因子：
6.9
通讯作者：
Zhuang, Xuliang
中科院分区：
文献类型：
--
作者：
Cheng, Yun;Zhang, Yang;Zhuang, Xuliang
N-酰基-高丝氨酸内酯(AHLS)作为群体感应信号，在许多蛋白质细菌中控制着许多细菌过程。本文报道了外源短链AHL对能利用硝酸盐进行好氧和厌氧生长的反硝化副球藻反硝化过程的影响。通过测量反硝化产物(包括硝酸盐、亚硝酸盐和一氧化二氮)以及硝酸盐、亚硝酸盐、一氧化氮和一氧化二氮还原酶的单个信使核糖核酸(MRNA)水平来监测这些细胞的反硝化活性。结果表明，在厌氧和好氧培养条件下，2MU/L C6-高丝氨酸内酯对菌体密度影响不大，N-己酰基-DL-高丝氨酸内酯对硝酸盐还原活性影响较小。但外源C6-HSL对脱氮假单胞菌亚硝酸还原酶和一氧化氮还原酶基因的转录均有显著影响，且在好氧条件下C6-HSL抑制了脱氮假单胞菌的N2O积累活性。相反，外源C6-HSL在厌氧条件下刺激了N2O的产生，这表明群体感应对反硝化的调节在N2O的释放中可能是重要的。(C)2016中国科学院生态环境科学研究中心。爱思唯尔出版社出版。
N-acyl-homoserine lactones (AHLs) serve as quorum-sensing signals, which control a number of bacterial processes in many proteobacteria. Here we report the effects of exogenous short-chain AHL on the denitrifying process of Paracoccus denitrificans, which are capable of aerobic and anaerobic growth by utilizing nitrate. The denitrification activity of these cells was monitored by measuring denitrification products (including nitrate, nitrite, and nitrous oxide), and the individual messenger ribonucleic acid (mRNA) levels of nitrate, nitrite, nitric oxide and nitrous oxide reductases. The results indicated that 2 mu mol/L C6-homoserine lactone (HSL) has little effect on cell density under either anaerobic or aerobic culture conditions, and the nitrate reduction activity appeared slightly affected by N-hexanoyl-DL-homoserine lactone (C6-HSL). However, exogenous C6-HSL significantly affected the transcription of nitrite reductase and nitric oxide reductase genes in P. denitrificans regardless of the presence of oxygen, and N2O accumulation activity in P. denitrificans was suppressed by C6-HSL under aerobic condition. In contrast, exogenous C6-HSL stimulated the production of N2O under anaerobic condition, suggesting that the regulation of denitrification by quorum sensing may be important in N2O release. (C) 2016 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B. V.