Development of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detection of avian influenza viruses in field specimens.
Development of reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detection of avian influenza viruses in field specimens.
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开发逆转录环介导的等温扩增（RT-LAMP）测定法，用于检测现场标本中的禽流感病毒。
DOI：
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发表时间：
2010
影响因子：
1.2
通讯作者：
Toshihiro Ito
中科院分区：
文献类型：
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作者：
S. Shivakoti;Hiroshi Ito;T. Murase;E. Ono;H. Takakuwa;T. Yamashiro;K. Otsuki;Toshihiro Ito
逆转录环介导等温扩增（RT-LAMP）是一种成熟的基因扩增方法，用于快速诊断各种传染病。为了检测禽流感病毒，特别是在现场标本中，设计了针对基质基因的特异性引物。使用三十四个病毒样本（包括来自不同地理区域的野生和家养禽类宿主的分离株）来确认引物的有效性。所有样本在不到1小时内均被确认呈阳性。 RT-LAMP 检测还能够检测各种现场样本（例如拭子、组织和粪便）中的禽流感病毒。这些结果表明，采用独特设计的引物开发的 RT-LAMP 检测在全面禽流感监测中具有潜在用途。
Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is an established gene amplification method for rapid diagnosis of various infectious diseases. In order to detect avian influenza viruses, particularly in field specimens, specific primers targeting the matrix gene were designed. Thirty-four virus samples, including isolates from wild and domestic avian hosts belonging to various geographical areas, were used to confirm the validity of the primers. All samples were confirmed to be positive in less than 1 hr. The RT-LAMP assay was also able to detect avian influenza virus in the various field samples, such as swabs, tissues, and feces. These results indicate that the developed RT-LAMP assay with uniquely designed primers is potentially useful in comprehensive avian influenza surveillance.