Glyco-stripping and glyco-swapping
Glyco-stripping and glyco-swapping
复制标题
DOI：
10.1021/cb600365q
复制
发表时间：
2006-01-01
影响因子：
4
通讯作者：
Liu, Hung-wen
中科院分区：
文献类型：
--
作者：
Melancon, Charles E., III;Thibodeaux, Christopher J.;Liu, Hung-wen
糖基转移酶（Glycosyltransferases，GT）是一种普遍存在的酶，它催化糖基从活化的供体转移到受体，在天然产物的生物合成、毒力和生物分子识别中起重要作用。糖对于天然产物的生物活性通常是至关重要的，并且用于产生这些化合物的不同糖型的方法是高度期望的。最近的研究表明，参与天然产物生物合成的几种GT催化可逆反应。使用糖交换和糖苷配基交换策略来利用这种可逆性以产生> 70种加利车霉素类似物。
Glycosyltransferases (GTs) are ubiquitous enzymes that catalyze the transfer of a sugar moiety from an activated donor to an acceptor and thus play important roles in natural product biogenesis, virulence, and biomolecular recognition. Sugars are often critical for bioactivity of natural products, and methodologies for creating diverse glycoforms of these compounds are highly desirable. A recent study demonstrates that several GTs involved in natural product biosynthesis catalyze reversible reactions. Sugar exchange and aglycon exchange strategies were used to exploit this reversibility to generate > 70 calicheamicin analogues.