The impact of spectral composition and light periodicity on the activity of two antioxidant enzymes (SOD and CAT) in the coral Favia favus
The impact of spectral composition and light periodicity on the activity of two antioxidant enzymes (SOD and CAT) in the coral Favia favus
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DOI：
10.1016/j.jembe.2005.06.018
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发表时间：
2006-01-10
影响因子：
2
通讯作者：
Dubinsky, Z
中科院分区：
文献类型：
--
作者：
Levy, O;Achituv, Y;Dubinsky, Z
在贫营养水域中，光谱大部分为蓝色，因此珊瑚组织和共生藻类对蓝光的生理和生化反应很重要。对两种自由基清除酶活性的波长依赖性的检查表明，在整个可见光（400-700 nm）范围内，蓝光范围（440-480 nm）的活性比红光（640680 nm）有所增加。这些数据首次显示了共生珊瑚蚕豆中光合作用的作用光谱与两种主要抗氧化酶活性之间的关系。研究发现，动物（宿主）对光光谱分布的酶响应高于虫黄藻，这可能是由于宿主组织内自由基的积累所致。此外，我们发现这些酶的活性在自然界中受到昼夜长度的影响，而在实验室中则受到光照持续时间的影响。在蓝色区域和整个 PAR 光谱下，还观察到颜料浓度随生长而变化，而使用快速重复率荧光计 (FRRF) 进行的荧光测量显示，在光谱的蓝色部分，西格玛截面减小，量子产率也降低。不同光谱下清除酶活性、色素浓度和荧光产量的这些变化对于珊瑚在高光辐射的浅水环境中的适应和生存至关重要。 (c) 2005 Elsevier B.V. 保留所有权利。
In oligotrophic waters the light spectrum is mostly blue, and therefore the physiological and biochemical responses to blue light occurring in the coral tissue and in the symbiotic algae are important. Examination of the wavelength dependence of two free radical scavenger enzyme activity revealed an increase in activity in the blue light range (440-480 nm) compared to the red (640680 nm) in the full visible light (400-700 nm) range. These data show for the first time the relationship between the action spectra of photosynthesis and the activity of two main antioxidant enzymes in the symbiotic coral Favia favus. It was found that in the animal (host) the enzyme response to the spectral distribution of light was higher than that of the zooxanthellae, probably due to accumulation of free radicals within the host tissue. Furthermore, we found that the activity of these enzymes is affected in nature by the length of the day and night, and in the laboratory, by the duration of the illumination. Changes in the pigment concentrations were also observed in response to growth under the blue region and the whole PAR spectrum, while fluorescence measurements with the fast repetition rate fluorometer (FRRF) showed a decrease in the sigma cross section and a decrease in the quantum yield also in the blue part of the spectrum. These changes of scavenger enzymes activity, pigment concentration and fluorescence yield at different light spectra are vital in acclimatization and survival of corals in shallow water environments with high light radiation. (c) 2005 Elsevier B.V. All rights reserved.