IDENTIFICATION AND CHARACTERIZATION OF 2 RECEPTORS FOR SCHISTOFLRFAMIDE ON LOCUST OVIDUCT
IDENTIFICATION AND CHARACTERIZATION OF 2 RECEPTORS FOR SCHISTOFLRFAMIDE ON LOCUST OVIDUCT
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DOI：
10.1016/0196-9781(94)90045-0
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发表时间：
1994-01-01
期刊：
影响因子：
3
通讯作者：
LANGE, AB
中科院分区：
文献类型：
--
作者：
WANG, ZX;ORCHARD, I;LANGE, AB
开发了一种使用[I-125][Y-1]血吸虫FLRF酰胺（YDVDHVFLRF酰胺）（血吸虫FLRF酰胺的碘化类似物）的体外结合试验，以证明和表征与蝗虫输卵管相关的血吸虫FLRF酰胺的推定受体。通过结合测定揭示了两种受体。高亲和力受体的Kd为9.52 +/- 1.15 × 10（-10）M，B-max为14.5 +/- 1.2 fmol/mg膜蛋白;低亲和力受体的Kd为1.86 +/- 0.16 × 10（-7）M，B-max为540 +/- 43 fmol/mg膜蛋白。与两种受体的结合均为可饱和、特异性和可逆的，并被[Y-1] SchistoFLRF酰胺、SchistoFLRF酰胺和ADVGHVFLRF酰胺竞争性抑制。结合是Ca 2+或Mg 2+依赖性的，并被2 mM EDTA完全抑制。受体表现出区域分布，与大多数的高亲和力受体与上侧输卵管，其中很少或没有接受神经支配，和大多数的低亲和力受体与下侧和共同的输卵管，其中接受广泛的神经支配。
An in vitro binding assay, using [I-125][Y-1]SchistoFLRFamide (YDVDHVFLRFamide), an iodinated analogue of SchistoFLRFamide, was developed to demonstrate and characterize putative receptors for SchistoFLRFamide associated with the locust oviduct. Two receptors were revealed by the binding assay. The high-affinity receptor had a K-d of 9.52 +/- 1.15 X 10(-10) M and a B-max of 14.5 +/- 1.2 fmol/mg membrane protein; the low-affinity receptor had a K-d of 1.86 +/- 0.16 x 10(-7) M and a B-max of 540 +/- 43 fmol/mg membrane protein. Binding to both receptors was saturable, specific, and reversible, and competitively inhibited by [Y-1]SchistoFLRFamide, SchistoFLRFamide, and ADVGHVFLRFamide. Binding was Ca2+ or Mg2+ dependent and was completely inhibited by 2 mM EDTA. The receptors showed a regional distribution, with the majority of the high-affinity receptors associated with the upper lateral oviducts, which receive little or no innervation, and most of the low-affinity receptors associated with the lower lateral and common oviducts, which receive extensive innervation.