Factors influencing infection and transmission of Anopheles gambiae densovirus (AgDNV) in mosquitoes
Factors influencing infection and transmission of Anopheles gambiae densovirus (AgDNV) in mosquitoes
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DOI：
10.7717/peerj.2691
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发表时间：
2016-11-09
期刊：
影响因子：
2.7
通讯作者：
Rasgon, Jason L.
中科院分区：
文献类型：
--
作者：
Barik, Tapan K.;Suzuki, Yasutsugu;Rasgon, Jason L.
冈比亚按蚊浓核病毒（Anopheles gambiae densovirus，AgDNV）是一种潜在的副转殖和基因转导微生物病原。冈比亚是撒哈拉以南非洲地区人类疟疾的主要传播媒介。了解AgDNV和An之间的相互作用。冈比亚按蚊是以基本和应用方式使用AgDNV进行按蚊基因操作的关键。在这里，我们测试了蚊子的年龄，性别，吸血状态的影响，并使用增强型绿色荧光蛋白（EGFP）报告AgDNV系统的水平传播的潜力。无论是蚊子感染的年龄，也没有喂养制度影响病毒滴度。雌蚊比雄蚊更容易感染病毒。尽管病毒滴度较低，但感染的雄性能够在交配期间通过性传播病毒给雌性，其中病毒与转移的精子一起定位在受精囊中。这些发现将有助于设计AgDNV为基础的策略来操纵冈比亚按蚊。
Anopheles gambiae densovirus (AgDNV) is a potential microbial agent for paratransgenesis and gene transduction in An. gambiae, the major vector of human malaria in sub-Saharan Africa. Understanding the interaction between AgDNV and An. gambiae is critical for using AgDNV in a basic and applied manner for Anopheles gene manipulation. Here, we tested the effects of mosquito age, sex, blood feeding status, and potential for horizontal transmission using an enhanced green fluorescent protein (EGFP) reporter AgDNV system. Neither mosquito age at infection nor feeding regime affected viral titers. Female mosquitoes were more permissive to viral infection than males. Despite low viral titers, infected males were able to venereally transmit virus to females during mating, where the virus was localized with the transferred sperm in the spermathecae. These findings will be useful for designing AgDNV-based strategies to manipulate Anopheles gambiae.