Aged Monkeys Fed a High-Fat/High-Sugar Diet Recapitulate Metabolic Disorders and Cardiac Contractile Dysfunction
Aged Monkeys Fed a High-Fat/High-Sugar Diet Recapitulate Metabolic Disorders and Cardiac Contractile Dysfunction
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DOI：
10.1007/s12265-021-10105-z
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发表时间：
2021-02
影响因子：
3.4
通讯作者：
Shuang-Shuang Zheng-Shuang;W. Tan;Xiang Li;Binglin Li;Baoyong Gong;W. Pyle;Jian Wu;Lei Li;Ting-Ting L
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文献类型：
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作者：
Shuang-Shuang Zheng-Shuang;W. Tan;Xiang Li;Binglin Li;Baoyong Gong;W. Pyle;Jian Wu;Lei Li;Ting-Ting L
老年非人灵长类动物（NHP）模型对于研究代谢性心脏病的病理机制和制定治疗策略具有重要价值。在这项研究中，老年雄性食蟹猴被喂食常规饮食或高脂/高糖饮食（HFSD）8个月。采用1H-NMR和血清生化检查诊断代谢紊乱，超声心动图评价心功能。我们的研究结果表明，在喂食HFSD的老年猴中，血清代谢谱发生了改变，与主动脉组织损伤、心脏重塑和收缩功能障碍一致。与正常饮食的老年猴相比，该老年猴模型显著增加了促炎细胞因子的表达，并改变了心脏中细胞内信号蛋白的表达和磷酸化。此外，这些动物证明了心脏肌丝蛋白的磷酸化增加，这与肌丝收缩性降低有因果关系。我们的结论是，老年猴模型喂养HFSD表现出代谢紊乱和心脏收缩功能障碍。
Aged nonhuman primate (NHP) models are of great value for studying the pathology of metabolic heart diseases and developing therapeutic strategies. In this study, aged male cynomolgus monkeys were fed a regular diet or a high-fat/high-sugar diet (HFSD) for 8 months. Metabolic disorders were diagnosed by1H-NMR and serum biochemistry, and cardiac function was evaluated by echocardiography. Our results showed that serum metabolic profiles were altered in aged monkeys fed a HFSD, in line with aortic tissue damage, cardiac remodeling, and contractile dysfunction. This aged monkey model significantly increased expression of proinflammatory cytokines and altered expression and phosphorylation of intracellular signaling proteins in the heart, as compared to aged monkeys on a regular diet. Furthermore, the animals demonstrated increased phosphorylation of cardiac myofilament proteins which are causatively associated with decreased myofilament contractility. We conclude that the aged monkey model fed a HFSD exhibits metabolic disorders and cardiac contractile dysfunction.