STRUCTURE OF MOUSE RIBOSOMAL-RNA PRECURSORS - COMPLETE SEQUENCE AND POTENTIAL FOLDING OF THE SPACER REGIONS BETWEEN 18S AND 28S RIBOSOMAL-RNA
STRUCTURE OF MOUSE RIBOSOMAL-RNA PRECURSORS - COMPLETE SEQUENCE AND POTENTIAL FOLDING OF THE SPACER REGIONS BETWEEN 18S AND 28S RIBOSOMAL-RNA
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DOI：
10.1093/nar/11.10.3375
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发表时间：
1983-01-01
影响因子：
14.9
通讯作者：
RAYNAL, F
中科院分区：
文献类型：
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作者：
MICHOT, B;BACHELLERIE, JP;RAYNAL, F
我们测定了小鼠核糖体RNA转录单位中分离成熟rRNA基因的区域的核苷酸全序列。这些内转录间隔区（ITS）在核糖体生物合成期间从rRNA前体切除。ITS 1位于18s和5.8s rRNA基因之间，全长999个核苷酸。ITS2位于5.8s和28s rRNA之间，长1089个核苷酸。两个间隔区都非常富含G + C，分别为70和74。小鼠的序列已经与其他可用的真核生物进行了比较：虽然与酵母或爪蟾没有明显的同源性，但小鼠和大鼠的ITS序列在很大程度上是保守的，同源片段散布着高度发散的片段。ITS 1与大鼠的同源性比ITS 2广泛得多。暂定二级结构模型提出的rRNA前体内的这些区域的折叠，它们在小鼠和大鼠密切相关。
We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which seperate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18s and 5.8s rRNA genes, is 999 nucleotides long. ITS 2, between 5.8s and 28s rRNA gnes, is 1089 nucleotides long. Both spacers are very rich in G + C,70 and 74 respectively. Mouse sequences have been compared with the other available eukaryotes : while no homology is apparent with yeast or xenopus, mouse and rat ITS sequences have been largely conserved, with homologous segmaents interspersed with highly divergent tracts. Homology with rat is much more extensive for ITS 1 than for ITS 2. Tentative secondary structure models are proposed for the folding of these regions within rRNA precursor;they are closely related in mouse and rat.