Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus.
Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus.
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癫痫持续状态大鼠海马区内皮细胞单核细胞激活多肽II表达的变化
DOI：
10.3892/ijmm.2020.4808
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发表时间：
2021-03
影响因子：
5.4
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Li C;Ma W;Zhao Y;Wang H
内皮单核细胞激活多肽II（EMAP II）是神经毒性损伤的敏感标志物，其表达在应激条件下显著增加，例如缺氧或凋亡。研究证实癫痫持续状态（stateepilepticus，SE）可引起脑内神经细胞广泛凋亡，SE的发生可使细胞处于缺氧状态。本研究旨在观察匹罗卡品致SE大鼠海马微血管内皮细胞EMAP Ⅱ表达、细胞数量及紧密连接蛋白水平的变化。免疫荧光和Western blot检测海马EMAP II、CD 31、闭合小带1（ZO-1）和occludin蛋白表达水平。结果发现，SE组中几乎75.6%的大鼠在毛果芸香碱给药后约44.7±18.8 min发生Racine IV-V期癫痫发作，24 h死亡率几乎为10.4%。SE组大鼠在SE后24 h内体重明显下降。免疫荧光染色显示，EMAP II在对照组大鼠的海马中的表达较低，然而，EMAP II阳性细胞的数量显着增加，在SE组从2 h至21 d。EMAP II蛋白表达趋势与免疫荧光染色结果一致。CD 31阳性微血管内皮细胞的数量从24 h到21 d与对照组的水平相比显著增加。ZO-1和occludin的蛋白表达在SE组中最显著降低。总之，本研究表明，SE模型大鼠海马EMAP II表达上调，可能促进血管生成，改变脑微血管内皮细胞TJ的完整性，增加CD 31阳性微血管内皮细胞的数量，降低ZO-1和occludin的表达。
Endothelial monocyte-activating polypeptide II (EMAP II) is a sensitive marker of neurotoxic injury, the expression of which increases significantly under conditions of stress, such as hypoxia or apoptosis. Studies have confirmed the extensive apoptosis of nerve cells in the brain following status epilepticus (SE), and the occurrence of SE can confer a hypoxic state on cells. The purpose of the present study was to observe the changes in the expression of EMAP II, and in the numbers and tight junction protein levels of micro-vascular endothelial cells in the hippocampus of rats with pilocarpine-induced SE. The protein expression levels of EMAP II, CD31, zonula occludens 1 (ZO-1) and occludin in the hippocampus were determined by immunofluorescence and western blot analyses. It was found that almost 75.6% of the rats in the SE group developed Racine stage IV-V seizures at approximately 44.7±18.8 min after the pilocarpine administration, and the 24-h mortality rate was almost 10.4%. The weight of the rats in the SE group was significantly decreased within 24 h following SE. Immunofluorescence staining revealed a low EMAP II expression in the hippo-campus of the rats in the control group; however, the numbers of EMAP II-positive cells were significantly increased in the SE group from 2 h to 21 days. The trend of EMAP II protein expression was consistent with that obtained with immunofluorescence staining. The numbers of CD31-positive microvascular endothelial cells were significantly increased from 24 h to 21 days compared with the levels in the control group. The protein expression of ZO-1 and occludin was most significantly decreased in the SE group. On the whole, the present study demonstrated that the expression of EMAP II in the rat hippocampus was upregulated in the SE model, which may promote angiogenesis and alter the TJ integrity of brain microvascular endothelial cells, with an increased number of CD31-positive microvascular endothelial cells and a decreased expression of ZO-1 and occludin.
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影响因子：
2.2
作者：
Glien, M;Brandt, C;Löscher, W
通讯作者：
Löscher, W
DOI：
10.1002/bdra.23234
发表时间：
2014-03-01
影响因子：
--
作者：
Lal, Charitharth Vivek;Schwarz, Margaret A.
通讯作者：
Schwarz, Margaret A.
ATP合酶α亚基在低剂量内皮单核细胞激活多肽-II诱导的血肿瘤屏障开放中的作用
影响因子：
4.4
作者：
Li, Zhen;Liu, Yun-hui;Liu, Li-bo
通讯作者：
Liu, Li-bo
影响因子：
15.9
作者：
Clauss, Matthias;Voswinckel, Robert;Petrache, Irina
通讯作者：
Petrache, Irina
影响因子：
3.3
作者：
Kim, J. Y.;Ko, A. -R.;Kang, T. -C.
通讯作者：
Kang, T. -C.