Determination of 18 phenolic acids in tobacco and rhizosphere soil by ultra high performance liquid chromatography combined with triple quadrupole mass spectrometry
Determination of 18 phenolic acids in tobacco and rhizosphere soil by ultra high performance liquid chromatography combined with triple quadrupole mass spectrometry
复制标题
超高效液相色谱-三重四极杆质谱法测定烟草及根际土壤中18种酚酸
DOI：
10.1002/jssc.201800819
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发表时间：
2019-02-01
影响因子：
3.1
通讯作者：
Yang, Guidi
中科院分区：
文献类型：
--
作者：
Chen, Yuxi;Chen, Wei;Yang, Guidi
建立了超高效液相色谱-三重四极杆质谱法测定烟草和土壤中游离和结合态酚酸的方法。以极性增强聚合物柱为固定相，甲醇为移动的相，采用简单的固相萃取法去除结合态酚酸，以氯仿为溶剂，采用液相萃取法去除游离态酚酸。用该方法在6 min内分离测定了重茬烤烟品种k326根际土壤中18种酚酸，回收率为82-107%，相对标准偏差（n=5）为1.1- 4.8%。结果表明，根际土壤、k326根和叶中游离酚酸分别占总酚酸的0- 9%、92- 100%和69-100%。对羟基苯甲酸、对香豆酸、香草酸、阿魏酸和胡萝卜酸是k326根际土壤中的主要酚酸，连作可导致k326根际土壤中除胡萝卜酸外的其它酚酸积累。酚酸类物质的测定为评价重茬烟植株和土壤中酚酸类物质的来源和特性提供了详细的信息。
An ultra high performance liquid chromatography with triple quadrupole mass spectrometry method for the determination of free and bound phenolic acids in tobacco plant and soil was developed. A simple solid-phase extraction, which used Polar Enhanced Polymer column as stationary phase and methanol as mobile phase, was used for the clean-up of bound phenolic acids, and a liquid-phase extraction using chloroformas solventwas used to purify free phenolic acids. With our method, 18 phenolic acids in rhizosphere soil of continuous cropping flue-cured cultivar k326 were separated and determined within 6 min with recoveries of 82-107% and relative standard deviations (n=5) of 1.1-4.8%. Results showed that free phenolic acids accounted for 0-9, 92-100, and 69-100% of total phenolic acids in rhizosphere soil, cultivar k326 roots and leaves, respectively. Results also revealed that p-hydroxybenzoic acid, p-coumaric acid, vanillic acid, ferulic acid, and syringic acid were the predominant phenolic acids in rhizosphere soil of cultivar k326, and continuous cropping of cultivar k326 in the same farmland could lead to the accumulation of these phenolic acids in soil except syringic acid. The determination of phenolic acids provided detailed information for evaluating their source and characteristics in continuous cropping tobacco plant and soil.