Interaction between limb bud ectoderm and mesoderm in the chick embryo. II. Experimental limb duplication
Interaction between limb bud ectoderm and mesoderm in the chick embryo. II. Experimental limb duplication
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鸡胚肢芽外胚层和中胚层之间的相互作用。
DOI：
10.1002/jez.1401320111
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发表时间：
1956
影响因子：
--
通讯作者：
E. Zwilling
中科院分区：
文献类型：
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作者：
E. Zwilling
我们对早期鸡胚肢芽之间的外胚层-中胚层交换的最初实验（Zwilling，'55）留下了许多未解决的问题。尽管外胚层有时很松散地置于中胚层之上（即桑德斯脊并不总是与最初依附于脊的中胚层接触），但仍能获得正常发育。肢体中胚层的其他部分可以对顶脊的生长刺激做出反应，或者两个肢体成分存在相互调节。试图通过消除最初的远端中胚层来解决这一点。在我们将要描述的实验中，这是通过两种方式实现的。实际上在一组肢芽中切掉了远端中胚层，并测试了中胚层的其余部分对顶脊的反应。在另一组中，来自单个肢芽的中胚层具有来自两个肢芽的顶脊。由于上述这类成功的实验可以从肢体重复的角度进行最好的评估，因此肢体发育的这一方面就成了本来文的中心主题。此外，我们还获得了有关肢芽调节能力的信息。简单地说，我们的结果毫无疑问地表明：（1）对结果的反应能力
Our initial experiments with ectoderm-mesoderm interchange between early chick embryo limb buds (Zwilling,'55) left a number of unresolved problems. Despite the fact that the ectoderm was sometimes placed upon the mesoderm quite loosely (ie Saunders' ridge did not always make contact with mesoderm which was originally subjacent to a ridge) normal development could be obtained. Either other parts of the limb mesoderm could respond to the outgrowth stimulus of the apical ridge or there was a mutual adjustment of the two limb components. An attempt was made to settle this point by the elimination of the initial distal mesoderm. In the experiments which w7e shall describe this was accomplished in two ways. The distal mesoderm was actually cut away in one group of limb buds, and the remainder of the mesoderm was tested for response to an apical ridge. In another group the mesoderm from a single limb bud was provided with apical ridges from tw7o limb buds. Since successful experiments of the kind mentioned above could be assessed best in terms of limb duplication this aspect of limb development becomes the central theme of the present communication. In addition we obtained information about the regulative capacity of limb buds. Briefly our results leave no doubt that:(1) The capacity to respond to the outgrowth