Transcription factor MafB is a marker of tumor-associated macrophages in both mouse and humans
Transcription factor MafB is a marker of tumor-associated macrophages in both mouse and humans
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DOI：
10.1016/j.bbrc.2019.10.125
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发表时间：
2020-01-15
影响因子：
3.1
通讯作者：
Hamada, Michito
中科院分区：
文献类型：
--
作者：
Yadav, Manoj Kumar;Inoue, Yuri;Hamada, Michito
转录因子MafB在巨噬细胞中特异性表达。我们最近已经证明MafB在体外表达于抗炎的交替激活的M2巨噬细胞中。肿瘤相关巨噬细胞（TAM）是M2型巨噬细胞的一个子集，可以促进免疫抑制活性，诱导血管生成，并促进肿瘤细胞增殖。为了检测MafB是否在TAM中表达，我们分析了MafB-GFP敲入杂合小鼠的刘易斯肺癌肿瘤中的绿色荧光蛋白（GFP）表达。FACS分析显示巨噬细胞标志物（F4/80、CD 11b、CD 68和CD 204）阳性的细胞中的GFP荧光。此外，用F4/80（+）GFP（+）和F4/80（+）GFP（-）分选的细胞进行的定量RT-PCR分析显示，GFP阳性巨噬细胞表达IL-10、Arg-1和TNF-α，已知它们在TAM中表达。这些结果表明MafB在TAM中表达。此外，使用抗MAFB抗体的免疫染色分析显示，MAFB在人肺癌样品中的CD 204-和CD 68-阳性巨噬细胞中表达。总之，MafB可以是小鼠和人肿瘤组织中TAM的合适标记物。(C)2019爱思唯尔公司All rights reserved.
The transcription factor MafB is specifically expressed in macrophages. We have recently demonstrated that MafB is expressed in anti-inflammatory alternatively activated M2 macrophages in vitro. Tumor-associated macrophages (TAMs) are a subset of M2 type macrophages that can promote immunosuppressive activity, induce angiogenesis, and promote tumor cell proliferation. To examine whether MafB express in TAMs, we analyzed green fluorescent protein (GFP) expression in Lewis lung carcinoma tumors of MafB-GFP knock-in heterozygous mice. FACS analysis demonstrated GFP fluorescence in cells positive for macrophage- markers (F4/80, CD11b, CD68, and CD204). Moreover, quantitative RT-PCR analysis with F4/80(+)GFP(+) and F4/80(+)GFP(-) sorted cells showed that the GFP-positive macrophages express IL-10, Arg-1, and TNF-alpha, which were known to be expressed in TAMs. These results indicate that MafB is expressed in TAMs. Furthermore, immunostaining analysis using an anti-MAFB antibody revealed that MAFB is expressed in CD204-and CD68-positive macrophages in human lung cancer samples. In conclusion, MafB can be a suitable marker of TAMs in both mouse and human tumor tissues. (C) 2019 Elsevier Inc. All rights reserved.