Studies of Intracorneal Distribution and Cytotoxicity of Quantum Dots: Risk Assessment of Eye Exposure
Studies of Intracorneal Distribution and Cytotoxicity of Quantum Dots: Risk Assessment of Eye Exposure
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DOI：
10.1021/tx100376n
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发表时间：
2011-02-01
影响因子：
4.1
通讯作者：
Tan, Hsin-Yuan
中科院分区：
文献类型：
--
作者：
Kuo, Tsung-Rong;Lee, Chia-Feng;Tan, Hsin-Yuan
角膜是纳米颗粒暴露和给药的潜在途径。在这项工作中，我们使用非侵入性双光子显微成像来研究覆盖有三种不同官能团的 CdSe/ZnS 核/壳量子点 (QD) 在角膜中的分布和渗透途径。在没有额外染色的情况下，双光子图像清楚地揭示了当角膜上皮屏障受损时，荧光量子点穿透并驻留在产生二次谐波的胶原基质的层间空间内。使用牛角膜基质细胞与所有三种量子点单独孵育的体外细胞毒性测试表明，随着量子点浓度和孵育时间的增加，细胞活力显着降低。结果还表明，在相对较低的浓度（5-20 nM）和较短的暴露时间（24-48 小时）下，特定的量子点对角膜基质细胞活力的影响高达 50%。此外，双光子成像表明，在体内小鼠模型中，QD 可以在角膜内保留长达 26 天。根据我们的体内和体外数据，我们得出结论，在角膜上皮屏障受损的情况下，量子点可以穿透并保留在角膜内足够长的时间，从而导致随之而来的细胞毒性。由于角膜擦伤在日常生活中相当常见，因此我们的工作引起了公众对眼睛接触纳米粒子的潜在风险的关注。
The cornea is a potential route of exposure and drug administration for nanoparticles. In this work, we use noninvasive two-photon microscopic imaging to study the distribution and permeability pathway of CdSe/ZnS core/shell quantum dots (QDs) capped with three different functional groups through the cornea. With no additional staining, the two-photon image clearly discloses that fluorescent QDs penetrate and reside within the interlamellar space of second harmonic generating collagenous stroma when the corneal epithelium barrier is injured. An in vitro cytotoxicity test using bovine corneal stromal cells incubated individually with all three kinds of QDs indicates that the cell viability decreases significantly as the QD concentration and incubation period increased. The results also show that the specific QDs influence corneal stromal cell viability up to a significant magnitude of 50% under a relatively low concentration (5-20 nM) and short exposure period (24-48 h). Furthermore, two-photon imaging shows that QDs can be retained within the cornea up to 26 days in an in vivo mouse model. On the basis of our in vivo and in vitro data, we conclude that QDs can penetrate and be retained within cornea long enough to cause consequential cytotoxicity, under the circumstance in which the corneal epithelium barrier is injured. Since corneal abrasion is quite a common situation in daily life, our work raises public attention to the potential risk of eye exposure to nanoparticles.