Characterization of matriptase expression in normal human tissues
Characterization of matriptase expression in normal human tissues
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DOI：
10.1177/002215540305100805
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发表时间：
2003-08-01
影响因子：
3.2
通讯作者：
Lin, CY
中科院分区：
文献类型：
--
作者：
Oberst, MD;Singh, B;Lin, CY
MATRIPTASE是一种II型跨膜丝氨酸蛋白酶，与上皮源性肿瘤的进展有关。该酶在正常上皮细胞生物学中的作用尚不清楚。Northern分析在富含上皮细胞的组织中检测到Mattritase mRNA，该蛋白在正常和癌变的乳腺、卵巢和结肠组织中表达。然而，有关Mattritase蛋白和信使核糖核酸在富含上皮的正常人体组织中的分布的系统分析尚未见报道。在这项研究中，我们使用组织微阵列和整个组织标本来表征该蛋白酶在各种正常人体组织中的表达。在大多数含上皮组织的上皮组分中均检测到显著的免疫反应和mRNA表达。在柱状、假复层柱状、立方和鳞状等所有类型的上皮中均可见Mattritase的表达。然而，在某些组织的显微解剖中观察到了不同的反应性空间分布。这表明，尽管Mattritase在许多类型的上皮细胞中广泛表达，但在选定的上皮细胞分化过程中，其在组织内的活性可能部分地在蛋白质和mRNA水平上受到调节。
Matriptase is a type II transmembrane serine protease that has been implicated in the progression of epithelium-derived tumors. The role of this protease in the biology of normal epithelial cells remains to be elucidated. Matriptase mRNA has been detected by Northern analysis in tissues rich in epithelial cells, and the protein is expressed in vivo in normal and cancerous breast, ovarian, and colon tissues. However, a systematic analysis of the distribution of matriptase protein and mRNA in normal human tissues rich in epithelium has not been reported. In this study we characterized the expression of the protease in a wide variety of normal human tissues using a tissue microarray and whole tissue specimens. Significant immunoreactivity and mRNA expression were detected in the epithelial components of most epithelium-containing tissues. Matriptase expression was found in all types of epithelium, including columnar, pseudostratified columnar, cuboidal, and squamous. Distinct spatial distributions of reactivity were observed in the microanatomy of certain tissues, however. This suggests that although matriptase is broadly expressed among many types of epithelial cells, its activity within a tissue may be regulated in part at the protein and mRNA levels during the differentiation of selected epithelia.