Comparison of different optomotor response readouts for visual testing in experimental autoimmune encephalomyelitis-optic neuritis
Comparison of different optomotor response readouts for visual testing in experimental autoimmune encephalomyelitis-optic neuritis
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DOI：
10.1186/s12974-020-01889-z
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发表时间：
2020-07-18
影响因子：
9.3
通讯作者：
Albrecht, Philipp
中科院分区：
文献类型：
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作者：
Hecker, Christina;Dietrich, Michael;Albrecht, Philipp
视运动反应越来越多地用于临床前研究，以评估啮齿动物的视觉功能。然而，对于特定的科学问题，最合适的测量协议并不总是建立。我们的目的是确定最佳参数的视觉功能分析实验性自身免疫性脑脊髓炎视神经炎（EAEON），多发性硬化症的动物模型。对比敏感度和空间频率的方差较小，重测信度较好。此外，这两个参数能够区分EAEON和对照组。与视网膜变性的相关性，通过光学相干断层扫描，免疫细胞的浸润，和临床残疾评分显示，空间频率是上级对比敏感度分析。因此，我们得出结论，空间频率测试更适合作为C57 B1/6 J EAEON小鼠的视敏度评估。此外，对比敏感度测量更耗时，可能导致动物更大的压力。
Optomotor response is increasingly used in preclinical research for evaluating the visual function in rodents. However, the most suitable measuring protocol for specific scientific questions is not always established. We aimed to determine the optimal parameters for visual function analysis in experimental autoimmune encephalomyelitis optic neuritis (EAEON), an animal model for multiple sclerosis. Contrast sensitivity as well as spatial frequency both had a low variance and a good test-retest reliability. Also, both parameters were able to differentiate between the EAEON and the control group. Correlations with the retinal degeneration, assessed by optical coherence tomography, the infiltration of immune cells, and the clinical disability score revealed that spatial frequency was superior to contrast sensitivity analysis. We therefore conclude that spatial frequency testing is better suited as visual acuity assessment in C57Bl/6 J EAEON mice. Furthermore, contrast sensitivity measurements are more time consuming, possibly leading to more stress for the animals.