Diversity and origin of Andean landraces of common bean
Diversity and origin of Andean landraces of common bean
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DOI：
10.2135/cropsci2001.413854x
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发表时间：
2001-05-01
期刊：
影响因子：
2.3
通讯作者：
Tohme, J
中科院分区：
文献类型：
--
作者：
Beebe, S;Rengifo, J;Tohme, J
属于安第斯基因库的菜豆（Phaseolus vulgaris L.）地方品种在植物和谷物形态以及农业生态适应方面具有显着的多样性。本研究的目的是确定安第斯地方品种大样本的遗传结构，并建立安第斯地方品种和可能作为驯化豆来源的野生豆种群之间的对应关系。使用扩增片段长度多态性 (AFLP) 技术结合多重对应分析 (MCA) 和算术平均未加权配对法 (UPGMA) 对代表安第斯豆三个公认品种并包括许多爆裂豆类型的总共 182 个地方品种进行了分析。还包括代表南美洲和中美洲野豆多样性的 29 个野豆种质。使用两组引物生成 189 个多态性 AFLP。 MCA 结果图表明，大多数地方品种种质形成单一的未分化群体，UPGMA 结合 bootstrapping 的分析证实了这一点。少数异常值呈现的条带表明中美洲豆类渗入。在南美洲的野生豆种群中，来自玻利维亚的野生豆种群与栽培豆最为接近，这表明玻利维亚可能是一个重要的初级驯化地。安第斯豆的遗传基础狭窄，强调需要扩大安第斯基因库的遗传基础。
Landraces of common bean (Phaseolus vulgaris L.) pertaining to the Andean gene pool are remarkably diverse in plant and grain morphology and agroecological adaptation. The objectives of this study were to determine the genetic structure of a large sample of Andean landraces, and to establish a correspondence between Andean landraces and wild bean populations that might have served as the source of domesticated bean. A total of 182 landraces representing the three recognized races of Andean bean and including many popping bean types were analyzed using amplified fragment length polymorphism (AFLP) technology with multiple correspondence analysis (MCA) and unweighted pair group method with arithmetic mean (UPGMA). Twenty-nine wild bean accessions representing the diversity of wild bean in South America and Middle America were also included. Two sets of primers were used to generate 189 polymorphic AFLP. The graph of the results of MCA indicated that most landrace accessions formed a single undifferentiated group, and analysis by UPGMA combined with bootstrapping confirmed this. A small number of outliers presented bands that suggested introgression from Mesoamerican beans. Among wild bean populations from South America, those from Bolivia graphed in closest proximity to the cultivated bean, suggesting that Bolivia might have been an important primary domestication site. The narrow genetic base of Andean beans emphasizes the need to broaden the genetic base of the Andean gene pool.