In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis.
In Vitro and In Vivo Efficacy of Albendazole Chitosan Microspheres with Intensity-Modulated Radiation Therapy in the Treatment of Spinal Echinococcosis.
复制标题
阿苯达唑壳聚糖微球联合调强放射治疗脊髓包虫病的体内外疗效
DOI：
10.1128/aac.00795-21
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发表时间：
2021-10-18
影响因子：
4.9
通讯作者：
Li F
中科院分区：
文献类型：
--
作者：
Wang S;Wang S;Wang W;Dai Y;Qiu Z;Ke W;Geng M;Li J;Li K;Ma Q;Li F
目前，临床上缺乏安全有效的治疗脊髓囊型包虫病的方法。最近的研究表明，阿苯达唑壳聚糖微球(ABZ-CS-MPS)和放射治疗具有一定的抗腹部包虫病的能力，因此本研究旨在比较ABZ-CS-MPS、调强放射治疗(IMRT)和联合治疗对脊髓包虫病的体内外治疗效果。首先，对原头节进行不同的处理，通过监测原头节活力的变化来评价它们各自的抗包虫病效果。然后，通过检测线粒体膜电位的变化、细胞凋亡蛋白的表达以及原头节细胞超微结构的变化来评价原头节细胞的凋亡状态。之后，我们建立了沙土鼠脊髓CE模型，并进一步应用B超和磁共振成像(MRI)技术对体内包虫的大小进行了评估。最后取囊泡称重，比较不同剂量组的抑制率。联合治疗18 后原头节病死率达90%以上，显示出最高的杀脊柱效果。此外，共聚焦成像、凋亡蛋白的表达和原头节的超微结构变化显示该组的凋亡率最高。在体内，联合用药的包囊抑制率也最高(61.4%)。综上所述，我们的研究结果表明，ABZ-CS-MPS联合调强放射治疗可能成为脊柱CE的一种新的治疗方案。我们还提供了一种利用B超和MRI技术评估包虫在动物体内生长和转移的方法。
Currently, there is a lack of clinically safe and effective treatment for spinal cystic echinococcosis (CE). Recent studies have shown that albendazole chitosan microspheres (ABZ-CS-MPs) and irradiation have certain anti-abdominal echinococcosis ability, so this study aims to compare the in vitro and in vivo therapeutic effects of ABZ-CS-MPs, intensity-modulated radiation therapy (IMRT), and combination therapy on spinal echinococcosis. First, protoscoleces were processed by different treatments to evaluate their respective antiechinococcosis effects by monitoring the viability change of protoscoleces. Then, the apoptotic status of protoscoleces was evaluated by detecting the changes of mitochondrial membrane potential, the expression of apoptosis proteins, and the ultrastructural alterations of protoscoleces. After that, we constructed a gerbil model of spinal CE and further applied B-ultrasound and magnetic resonance imaging (MRI) technology to assess the size of hydatid in vivo. Finally, the cysts were obtained and weighed to compare the inhibition rate in different groups. The combined therapy increased protoscoleces mortality to over 90% after 18 days, which showed the highest scolicidal effect. Moreover, confocal imaging, expression of apoptotic proteins, and ultrastructural changes of protoscoleces showed the highest apoptotic rate in this group. In vivo, the combination treatment also exhibited the highest cyst inhibition rate (61.4%). In conclusion, our results showed that ABZ-CS-MPs combined with IMRT could be a new treatment option for spinal CE. We also provided a method to evaluate the growth and metastasis of hydatid in animals with B-ultrasound and MRI technologies.