Biocompatibility of polysebacic anhydride microparticles with chondrocytes in engineered cartilage.
Biocompatibility of polysebacic anhydride microparticles with chondrocytes in engineered cartilage.
复制标题
聚癸二酸酐微粒与工程软骨中软骨细胞的生物相容性。
DOI：
10.1016/j.colsurfb.2015.08.040
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Somasundaran,Ponisseril
中科院分区：
文献类型：
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作者：
Ponnurangam,Sathish;O'Connell,GraceD;Hung,ClarkT;Somasundaran,Ponisseril
开发临床相关的工程化软骨的主要挑战之一是克服由于在构建体的外围的细胞外基质的渐进加工而导致的有限的营养扩散。大通道已被用于减少营养路径长度;然而，通道在培养中几周内被基质堵塞，减少了营养扩散。或者，微粒可以嵌入整个支架中以提供局部营养物递送。在这项研究中，我们评估了生物相容性的聚癸二酸酐（PSA）聚合物和有效性的PSA为基础的微粒短期交付的营养物质在工程软骨。浓度高达2 mg/mL的PSA基微粒与幼龄牛软骨细胞具有生物相容性;然而，在20 mg/mL时观察到细胞毒性。高浓度下的细胞毒性可能是由于PSA降解产物的细胞内蓄积和导致的脂毒性。PSA的细胞毒性在牛血清白蛋白的存在下被部分逆转。总之，这项研究的结果表明，浓度依赖性的生物相容性的PSA为基础的微粒和潜在的应用作为一种营养输送车辆，可以嵌入到支架组织工程。
One of main challenges in developing clinically relevant engineered cartilage is overcoming limited nutrient diffusion due to progressive elaboration of extracellular matrix at the periphery of the construct. Macro-channels have been used to decrease the nutrient path-length; however, the channels become occluded with matrix within weeks in culture, reducing nutrient diffusion. Alternatively, microparticles can be imbedded throughout the scaffold to provide localized nutrient delivery. In this study, we evaluated biocompatibility of polysebacic anhydride (PSA) polymers and the effectiveness of PSA-based microparticles for short-term delivery of nutrients in engineered cartilage. PSA-based microparticles were biocompatible with juvenile bovine chondrocytes for concentrations up to 2 mg/mL; however, cytotoxicity was observed at 20 mg/mL. Cytotoxicity at high concentrations is likely due to intracellular accumulation of PSA degradation products and resulting lipotoxicity. Cytotoxicity of PSA was partially reversed in the presence of bovine serum albumin. In conclusion, the findings from this study demonstrate concentration-dependent biocompatibility of PSA-based microparticles and potential application as a nutrient delivery vehicle that can be imbedded in scaffolds for tissue engineering.