Fractionating soluble microbial products in the activated sludge process
Fractionating soluble microbial products in the activated sludge process
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活性污泥工艺中可溶性微生物产物的分级
DOI：
10.1016/j.watres.2009.12.025
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发表时间：
2010-04-01
期刊：
影响因子：
12.8
通讯作者：
Yu, Han-Qing
中科院分区：
文献类型：
--
作者：
Ni, Bing-Jie;Zeng, Raymond J.;Yu, Han-Qing
可溶性微生物产物（SMP）是微生物生长和腐烂产生的有机化合物库，通常是生物处理过程废水中可溶性有机物的主要成分。在这项工作中，使用综合化学分析和数学方法对活性污泥中的 SMP 进行了表征、分级和定量。 SMP 中底物利用过程中产生的利用相关产物 (UAP) 是分子量 (MW) 低于 290 kDa 的碳质化合物，与生物质相关产物 (BAP) 分开进行定量。 BAP主要是分子量在290-5000 kDa范围内的细胞大分子，并首次进一步分为微生物生长期产生的分子量为1000 kDa的生长相关BAP（GBAP）和内源相产生的分子量为4500 kDa的内源相关BAP（EBAP）。实验和建模结果表明，UAP 可以被活性污泥利用，并且 BAP 会在系统中积累。 GBAP 和 EBAP 具有不同的细胞外聚合物水解形成速率和不同的生物降解动力学。这项研究有助于更好地了解 SMP 形成机制，并对后续废水处理有用。 (C) 2009 Elsevier Ltd. 保留所有权利。
Soluble microbial products (SMP) are the pool of organic compounds originating from microbial growth and decay, and are usually the major component of the soluble organic matters in effluents from biological treatment processes. In this work, SMP in activated sludge were characterized, fractionized, and quantified using integrated chemical analysis and mathematical approach. The utilization-associated products (UAP) in SMP, produced in the substrate-utilization process, were found to be carbonaceous compounds with a molecular weight (MW) lower than 290 kDa which were quantified separately from biomass-associated products (BAP). The BAP were mainly cellular macromolecules with an MW in a range of 290-5000 kDa, and for the first time were further classified into the growth-associated BAP (GBAP) with an MW of 1000 kDa, which were produced in the microbial growth phase, and the endogeny-associated BAP (EBAP) with an MW of 4500 kDa, which were generated in the endogenous phase. Experimental and modeling results reveal that the UAP could be utilized by the activated sludge and that the BAP would accumulate in the system. The GBAP and EBAP had different formation rates from the hydrolysis of extracellular polymeric substances and distinct biodegradation kinetics. This study provides better understanding of SMP formation mechanisms and becomes useful for subsequent effluent treatment. (C) 2009 Elsevier Ltd. All rights reserved.