Metabonomic studies of human hepatocellular carcinoma using high-resolution magic-angle spinning 1H NMR spectroscopy in conjunction with multivariate data analysis
Metabonomic studies of human hepatocellular carcinoma using high-resolution magic-angle spinning 1H NMR spectroscopy in conjunction with multivariate data analysis
复制标题
使用高分辨率魔角旋转 1H NMR 波谱结合多变量数据分析对人肝细胞癌进行代谢组学研究
DOI：
10.1021/pr070063h
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发表时间：
2007-01-01
影响因子：
4.4
通讯作者：
Deng, Feng
中科院分区：
文献类型：
--
作者：
Yang, Yongxia;Li, Chenglong;Deng, Feng
已采用高分辨率魔角旋转（MAS）H-1核磁共振光谱来表征代谢物组成（即，代谢物组）与主成分分析（PCA）相结合。结果表明：（a）低级别肝癌和高级别肝癌的代谢组与癌旁非受累组织的代谢组有明显差异;（B）低级别肝癌与高级别肝癌的代谢组有明显差异。与未受累的邻近肝组织相比，HCC肿瘤的乳酸盐、谷氨酸盐、谷氨酰胺、甘氨酸、亮氨酸、丙氨酸、胆碱代谢物和磷酸乙醇胺（PE）水平升高，但甘油三酯、葡萄糖和糖原水平下降。高级别HCC中乳酸、氨基酸（包括谷氨酸、谷氨酰胺、甘氨酸、亮氨酸和丙氨酸）、胆碱和磷酸乙醇胺（PE）的水平高于低级别HCC，但PC、GPC、甘油三酯、葡萄糖和糖原的水平低于低级别HCC。与非炎性、低级别HCC肿瘤相比，炎性、低级别HCC肿瘤显示乳酸、磷酸胆碱（PC）和甘油磷酸胆碱（GPC）的统计学显著增加。HCC肿瘤的坏死导致可观察到的甘油三酯水平急剧增加，其信号主导其H-1 NMR谱。这些结果表明，HRMAS结合PCA提供了一个有用的工具，了解肿瘤生物化学和肝肿瘤组织的分类，这种工具也可能有一些潜力，肝肿瘤的诊断和预后，即使一些其他疾病的过程中存在。
High-resolution magic-angle spinning (MAS) H-1 nuclear magnetic resonance spectroscopy has been employed to characterize the metabolite composition (i.e., metabonome) of the human hepatocellular carcinoma (HCC) tumor in combination with principal component analysis (PCA). The results showed that (a) the metabonomes of both low-grade HCC and high-grade HCC tumors differ markedly from that of the adjacent non-involved tissues; and (b) low-grade HCC tumors have clear differences in metabonome from that of the high-grade HCC tumors. Compared with the non-involved adjacent liver tissues, HCC tumors had elevated levels of lactate, glutamate, glutamine, glycine, leucine, alanine, choline metabolites, and phosphorylethanolamine (PE), but declined levels of triglycerides, glucose, and glycogen. The levels of lactate, amino acids including glutamate, glutamine, glycine, leucine and alanine, choline and phosphorylethanolamine (PE) were higher but the levels of PC, GPC, triglycerides, glucose, and glycogen were lower in high-grade HCC than in low-grade HCC tumors. Compared with non-cirrhotic, low-grade HCC tumors, the cirrhotic, low-grade HCC tumors showed statistically significant increases in lactate, phosphocholine (PC), and glycerophosphocholine (GPC). The necrosis in HCC tumors resulted in a drastic increase in the levels of observable triglycerides, signals of which dominated their H-1 NMR spectra. These results indicated that HRMAS combined with PCA offers a useful tool for understanding the tumor biochemistry and classification of liver tumor tissues; such tool may also have some potential for liver tumor diagnosis and prognosis even when some other disease processes are present.