Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast
Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast
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DOI：
10.1091/mbc.e05-09-0900
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Mabuchi, I
中科院分区：
文献类型：
--
作者：
Nakano, K;Mabuchi, I
研究了肌动蛋白解聚因子（ADF）/cofilin家族蛋白Adf 1在裂殖酵母细胞胞质分裂中的作用。Adf 1是通过在细胞末端解聚肌动蛋白而在分裂位点积累肌动蛋白、通过切断肌动蛋白丝组装收缩环以及维持一旦形成的收缩环所必需的。遗传和细胞学分析表明，它与profilin和capping蛋白在有丝分裂肌动蛋白细胞骨架的重组。此外，意外地发现Adf 1和肌球蛋白-II也在组装收缩环中协作。原肌球蛋白被证明拮抗Adfl在收缩环中的功能。我们认为，收缩环的形成和维持是通过这些蛋白质的平衡协作来实现的。
The role of the actin-depolymerizing factor (ADF)/cofilin-family protein Adf1 in cytokinesis of fission yeast cells was studied. Adf1 was required for accumulation of actin at the division site by depolymerizing actin at the cell ends, assembly of the contractile ring through severing actin filaments, and maintenance of the contractile ring once formed. Genetic and cytological analyses suggested that it collaborates with profilin and capping protein in the mitotic reorganization of the actin cytoskeleton. Furthermore, it was unexpectedly found that Adf1 and myosin-II also collaborate in assembling the contractile ring. Tropomyosin was shown to antagonize the function of Adfl in the contractile ring. We propose that formation and maintenance of the contractile ring are achieved by a balanced collaboration of these proteins.