Neuron Culture from Mouse Superior Cervical Ganglion.
Neuron Culture from Mouse Superior Cervical Ganglion.
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DOI：
10.21769/bioprotoc.1035
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发表时间：
2014-01
期刊：
影响因子：
0.8
通讯作者：
Marisa Z. Jackson;W. Tourtellotte
中科院分区：
文献类型：
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作者：
Marisa Z. Jackson;W. Tourtellotte
啮齿动物颈上神经节(SCG)是研究交感神经系统(SNS)体外发育和生长机制的有用和容易获得的神经元来源。出生后早期动物的交感神经系统(SNS)经历了大量的重塑和发育；因此，从这个年龄段的小鼠身上提取的神经元在原位移除后可以重新生长并建立突触连接。SCG的固定位置和大小使其成为快速分离和分离的理想选择。这里描述的方案详细说明了SCG神经元的解剖、培养和分化的要求。该方法适用于从胚胎晚期到出生后3天左右的神经元培养。下面讨论的培养技术利用玻璃片对固定细胞进行显微镜检查。
The rodent superior cervical ganglion (SCG) is a useful and readily accessible source of neurons for studying the mechanisms of sympathetic nervous system (SNS) development and growth in vitro. The sympathetic nervous system (SNS) of early postnatal animals undergoes a great deal of remodeling and development; thus, neurons taken from mice at this age are primed to re-grow and establish synaptic connections after in situ removal. The stereotypic location and size of the SCG make it ideal for rapid isolation and dissociation. The protocol described here details the requirements for the dissection, culture and differentiation of SCG neurons. The protocol is suitable for culturing neurons from late embryonic gestation to approximately postnatal day 3. The culture technique discussed below utilizes glass coverslips for the microscopic examination of fixed cells.