Why don't biologists use SIMS? A critical evaluation of imaging MS
Why don't biologists use SIMS? A critical evaluation of imaging MS
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DOI：
10.1016/j.apsusc.2006.02.134
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发表时间：
2006-07-30
影响因子：
6.7
通讯作者：
Piersma, S. R.
中科院分区：
文献类型：
--
作者：
Heeren, R. M. A.;McDonnell, L. A.;Piersma, S. R.
二次离子质谱通常用于研究许多不同类型的复杂表面。然而，与MALDI和ESI-MS相比，西姆斯在生物学或生物医学研究中没有产生重大影响。该技术的关键特征，即高空间分辨率，高检测效率的离子跨越宽的m/z范围，表面灵敏度和高扫描速率似乎理想地匹配在细胞水平上提出的几个问题。到目前为止，西姆斯在生物竞技场上只取得了有限的成功。为什么会这样，需要什么来改变这一点？本文将批判性地回顾西姆斯在生物医学研究中的进展和有用性，并将其与其他方法进行比较，这些方法为具有生物学兴趣的研究人员提供空间分辨的分子信息。我们将证明，西姆斯的各种化身所产生的信息的类型是强烈依赖于样品制备和表面条件，这些现象只是知之甚少。现代的方法，如集束枪的发展，ME-SIMS，金涂层和MALDI柱头成像的西姆斯仪器可能会改变西姆斯的看法是一种工具，半导体制造商和物理学家，并可能说服生物学家使用这些创新的质谱成像工具。(c)2006年由Elsevier B. V.出版
Secondary ion mass spectrometry is commonly used to study many different types of complex surfaces. Yet, compared with MALDI and ESI-MS, SIMS has not made a significant impact in biological or biomedical research. The key features of the technique, namely high spatial resolution, high detection efficiency of ions spanning a wide m/z range, surface sensitivity and the high scan rates seem to match ideally with several questions posed at the cellular level. To this date, SIMS has had only limited success in the biological arena. Why is this and what is needed to change this? This discussion paper will critically review the advances and the usefulness of SIMS in biomedical research and compare it to other approaches that offer spatially resolved molecular information available to a researcher with a biological interest. We will demonstrate that the type of information generated by the various incarnations of SIMS is strongly dependent on sample preparation and surface condition and these phenomena are only poorly understood. Modern approaches such as the cluster gun developments, ME-SIMS, gold coating and MALDI stigmatic imaging on a SIMS instrument might change the perception of SIMS being a tool for semiconductor manufacturers and physicists, and might persuade biologists to use these innovative mass spectrometric imaging tools. (c) 2006 Published by Elsevier B.V.