POLYMERIZATION OF THE 9TH COMPONENT OF COMPLEMENT (C9) - FORMATION OF POLY(C9) WITH A TUBULAR ULTRASTRUCTURE RESEMBLING THE MEMBRANE ATTACK COMPLEX OF COMPLEMENT
POLYMERIZATION OF THE 9TH COMPONENT OF COMPLEMENT (C9) - FORMATION OF POLY(C9) WITH A TUBULAR ULTRASTRUCTURE RESEMBLING THE MEMBRANE ATTACK COMPLEX OF COMPLEMENT
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DOI：
10.1073/pnas.79.2.574
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
TSCHOPP, J
中科院分区：
文献类型：
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作者：
PODACK, ER;TSCHOPP, J
补体的第 9 个成分 (C9) 具有明显的聚合倾向。将纯化的人 C9 在 37°C 下孵育 64 小时后，C9 聚合物 [聚 (C9)] 在 Veronal 缓冲盐水中自发形成。 C 或 46-56°C 2 小时内C.在37℃下形成的Poly(C9)。通过 EM 将 C 可视化为内径为 110 埃的管状结构。长度为 160 ANG。其超微结构表明其为十二聚体组成，类似于补体的膜攻击复合体。管状结构的较宽端由.apprxeq 形成。 30 埃厚的圆环，内径和外径均为 110 埃。分别为220.ANG.和220.ANG.。因为poly(C9)内C9的尺寸是160倍。 55.ANG。 （最大）和 20 ANG。 （最小）并且因为单体 C9 的尺寸约为 80 倍。 55.ANG.，单体C9可能在聚合成小管的过程中展开。 C9在37℃处理1小时后也发生聚合。 C 用 0.6 M 胍.cntdot.HCl、0.1 M 辛基葡萄糖苷或 1.5% 脱氧胆酸钠。胍.cntdot.HCl 诱导的 C9 聚合物由细长的高度弯曲链 55-80 .ANG 组成。宽，表明这些聚合物是由尚未展开的球状 C9 形成的。
The 9th component of complement (C9) has a marked propensity to polymerize. C9 polymers [poly(C9)] formed spontaneously in Veronal-buffered saline upon incubation of purified human C9 for 64 h at 37.degree. C or within 2 h at 46-56.degree. C. Poly(C9) formed at 37.degree. C was visualized by EM as a tubular structure with an internal diameter of 110 .ANG. and a length of 160 .ANG.. Its ultrastructure suggested a dodecameric composition and resembled that of the membrane attack complex of complement. The wider end of the tubular structure was formed by an .apprxeq. 30-.ANG.-thick torus with inner and outer diameters of 110 .ANG. and 220 .ANG., respectively. Because the dimensions of C9 within poly(C9) were 160 .times. 55 .ANG. (maximal) and 20 .ANG. (minimal) and because monomeric C9 has dimensions of approximately 80 .times. 55 .ANG., monomeric C9 probably unfolds during polymerization into tubules. Polymerization also occurred upon treatment of C9 for 1 h at 37.degree. C with 0.6 M guanidine.cntdot.HCl, 0.1 M octyl glucoside or 1.5% sodium deoxycholate. Guanidine.cntdot.HCl-induced C9 polymers consisted of elongated highly curved strands 55-80 .ANG. wide, suggesting that these polymers were formed by globular C9 that had not unfolded.