Motif-Programmed Artificial Extracellular Matrix
Motif-Programmed Artificial Extracellular Matrix
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DOI：
10.1021/bm800638z
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发表时间：
2008-11-01
影响因子：
6.2
通讯作者：
Shiba, Kiyotaka
中科院分区：
文献类型：
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作者：
Kokubun, Katsutoshi;Kashiwagi, Kenji;Shiba, Kiyotaka
基序编程是一种通过组合功能肽基序来创造人造蛋白质的方法。这种方法特别适合于开发细胞和无机材料之间的连接分子。在这里，我们描述了我们通过编程创建两个基序的人造蛋白质，一个是自然细胞附着基序(RGD)，另一个是人工钛结合基序(min TBP-1)。所产生的蛋白可可逆地结合钛和MC3T3-E1成骨样细胞。此外，虽然与钛的相互作用不是共价的，但这些蛋白概括了纤维连接蛋白的几个功能，因此可以作为钛材料上的人工ECM。由于该基序编程系统可以很容易地扩展以产生具有其他生物功能和材料特性的人造蛋白质，因此它在组织工程和再生医学中的应用应该是非常有用的。
Motif-programming is a method for creating artificial proteins by combining functional peptide motifs in a combinatorial manner. This method is particularly well suited for developing liaison molecules that interface between cells and inorganic materials. Here we describe our creation of artificial proteins through the programming of two motifs, a natural cell attachment motif (RGD) and an artificial Ti-binding motif (min TBP-1). The created proteins were found to reversibly bind Ti and to bind MC3T3-E1 osteoblast-like cells. Moreover, although the interaction with Ti was not covalent, the proteins recapitulated several functions of fibronectin, and thus, could serve as an artificial ECM on Ti materials. Because this motif-programming system could be easily extended to create artificial proteins having other biological functions and material specificities, it should be highly useful for application to tissue engineering and regenerative medicine.